El-Aziz Heba Abd, Hammouda Nada E, Belal Fathallah, Elama Heba Samir
Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Mansoura University, Mansoura, 35516, Egypt.
Unit of Drug Analysis, Faculty of Pharmacy, Mansoura University, Mansoura, 35516, Egypt.
J Fluoresc. 2024 Oct 24. doi: 10.1007/s10895-024-03962-9.
Chrysin is a plant flavonoid that has different therapeutic effects as anti-inflammatory, anti-cancer, anti-oxidant, and immune booster. Spectrofluorimetry has received a lot of interest lately because of its ecological greenness and analytical performance. This approach employed the native fluorescence of chrysin at 339 nm following excitation at 231 nm in distilled water. Modern advances in analytical chemistry have been used to lessen occupational and environmental concerns by employing distilled water as a dilution solvent through method development and application. The approach was found to be excellent green supported by eco-scale score of 97 and 0.94 AGREE rating, in addition to an overall whiteness score of 88.80. The design aimed to analyze chrysin in raw materials, Chrysin® capsules and human plasma. The method was linear over 0.5-7.0 ng mL chrysin, with LOD of 0.06 ng mL and LOQ of 0.20 ng mL. The offered method was effectively applied for determination of chrysin in the commercial capsules Chrysin® and spiked human plasma samples with average recoveries of 99.76% and 99.98%, respectively for capsules and spiked human plasma. Up to date, no spectrofluorimetric method has been described for chrysin analysis, then, this presented an opportunity to develop a sensitive, quick, reliable, environmentally friendly, and valid fluorescence-based method.
白杨素是一种植物黄酮类化合物，具有抗炎、抗癌、抗氧化和增强免疫力等不同的治疗作用。荧光分光光度法因其生态绿色性和分析性能最近受到了广泛关注。该方法利用白杨素在蒸馏水中于231nm激发后在339nm处的天然荧光。分析化学的现代进展已被用于通过方法开发和应用，以蒸馏水作为稀释溶剂来减少职业和环境方面的担忧。该方法被认为是优秀的绿色方法，生态规模评分为97，AGREE评分为0.94，此外总体白度评分为88.80。该设计旨在分析原材料、Chrysin®胶囊和人体血浆中的白杨素。该方法在0.5 - 7.0 ng/mL白杨素范围内呈线性，检测限为0.06 ng/mL，定量限为0.20 ng/mL。所提供的方法有效地应用于商业Chrysin®胶囊和加标人体血浆样品中白杨素的测定，胶囊和加标人体血浆的平均回收率分别为99.76%和99.98%。到目前为止，尚未有用于白杨素分析的荧光分光光度法被描述，因此，这为开发一种灵敏、快速、可靠、环境友好且有效的基于荧光的方法提供了机会。
