Strause L G, Hegenauer J, Saltman P, Cone R, Resnick D
J Nutr. 1986 Jan;116(1):135-41. doi: 10.1093/jn/116.1.135.
Experiments were designed to produce biochemical changes in both serum and bone in rats by long-term dietary deficiencies of manganese (Mn) and copper (Cu). Weanling rats were fed one of three diets: 1) normal Mn and Cu (N), 2) low Mn and Cu (L) and 3) depleted Mn and normal Cu (D). After 12 mo, serum Ca and P were significantly greater in the D rats than the N rats (134, 50 mg/L and 103, 39 mg/L, respectively). Serum and femur Mn levels were lower in D rats than in normal rats. Serum and femur Cu levels were lower in L rats than N rats. Femur Ca concentrations were significantly lower in D rats than in N rats (180 and 272 mg/g, respectively). Radiographic differences could be detected in some rats fed the L and D diets. In the two deficient groups, increased serum Ca was negatively correlated with bone Ca (r = -0.69; P less than 0.01). This biochemical association may represent alterations in regulatory control of Ca at the level of the bone (decreased mineralization) combined with an increase in bone resorption. The effect of long-term dietary deficiencies in Mn and Cu should be considered in human bone metabolism.
实验旨在通过长期饮食中缺乏锰（Mn）和铜（Cu），使大鼠血清和骨骼产生生化变化。将断奶大鼠分为三组喂食三种不同的饮食：1）正常锰和铜（N），2）低锰和铜（L），3）缺锰和正常铜（D）。12个月后，D组大鼠血清钙和磷显著高于N组大鼠（分别为134、50mg/L和103、39mg/L）。D组大鼠血清和股骨锰水平低于正常大鼠。L组大鼠血清和股骨铜水平低于N组大鼠。D组大鼠股骨钙浓度显著低于N组大鼠（分别为180和272mg/g）。在一些喂食L和D饮食的大鼠中可检测到放射学差异。在两个缺乏组中，血清钙升高与骨钙呈负相关（r = -0.69；P < 0.01）。这种生化关联可能代表了骨水平上钙调节控制发生改变（矿化减少），同时骨吸收增加。在人类骨代谢中应考虑长期饮食中缺乏锰和铜的影响。
