Farhadian Nasrin, Miresmaeili Amirfarhang, Azar Ramin, Zargaran Massoumeh, Moghimbeigi Abbas, Soheilifar Sanaz
Associate Professor, Department of Orthodontics, Faculty of dentistry, Hamadan University of Medical Sciences, Hamadan, Iran.
Assistant Professor, Department of Orthodontics, Faculty of dentistry, Hamadan University of Medical Sciences, Hamadan, Iran.
J Dent (Tehran). 2015 Jan;12(1):39-48.
After maxillary expansion, a long period of retention is necessary to prevent early relapse. Therefore, it is beneficial to accelerate bone formation in the expanding midpalatal suture to reduce relapse. This study was designed to evaluate the effect of dietary vitamin C on osteogenesis of rat midpalatal suture during expansion.
Fifty-four male Wistar rats were randomly divided into three groups, each with a control and an experimental subgroup. An open-loop spring was bonded to maxillary incisors of each animal to expand the premaxillary suture. Experimental groups received dietary vitamin C in their water. The rats in the three groups were sacrificed at three, nine or 17-day intervals after bonding the spring. Then, the premaxilla was dissected and sections were made and stained with hematoxylin and eosin and osteopontin marker. Osteoblasts and osteoclasts were counted in the suture. Two-way ANOVA and the Mann-Whitney-U test were used for analyzing the data.
After three days, the number of osteoblasts was significantly higher in the vitamin C group but after nine days it was significantly higher in the control group and after seventeen days there were no significant differences between the groups. Osteoclast counts were not significantly different between vitamin C and control groups.
Vitamin C had a positive effect on osteogenesis at the beginning of bone formation in the expanding suture, but after nine days it had a negative effect on suture osteogenesis in rats.
上颌扩弓后，需要长时间保持以防止早期复发。因此，促进腭中缝扩张部位的骨形成以减少复发是有益的。本研究旨在评估膳食维生素C对上颌扩弓期间大鼠腭中缝骨生成的影响。
54只雄性Wistar大鼠随机分为三组，每组又分为对照亚组和实验亚组。将开环弹簧粘结到每只动物的上颌切牙上以扩宽前颌缝。实验组在饮水中摄入膳食维生素C。在粘结弹簧后的第3、9或17天，处死三组大鼠。然后，解剖前颌骨并制作切片，用苏木精-伊红和骨桥蛋白标记物染色。对缝内的成骨细胞和破骨细胞进行计数。采用双向方差分析和曼-惠特尼-U检验分析数据。
3天后，维生素C组的成骨细胞数量显著更高，但9天后对照组的成骨细胞数量显著更高，17天后两组之间无显著差异。维生素C组和对照组的破骨细胞计数无显著差异。
维生素C在扩弓缝骨形成开始时对骨生成有积极作用，但9天后对大鼠缝骨生成有消极作用。
