Heart rate variability (HRV) is the change in time intervals between heart beats, reflecting the autonomic nervous system's ability to adapt to psychological and physiological demands. Slow breathing enhances parasympathetic activity, increasing HRV. , a yoga breathing technique, affords the conscious regulation of respiration frequency. This study aimed to characterize HRV, blood pressure and peripheral oxygen saturation of basic yoga breathing slow techniques with regular yoga practitioners. In total, 45 yoga practitioners were included in the study (including 7 males, mean age of 54.04 ± 11.97 years) with varying levels of yoga experience (minimum 3 months, maximum 37 years). Participants performed three breathing conditions: baseline (control) and two yoga techniques (abdominal () and complete ()) breathing, each for 10 min in the supine position (i.e., ). For each condition, respiratory frequency, heart rate (HR), blood pressure and peripheral oxygen levels were collected. The findings revealed that both abdominal and complete yoga breathing techniques promoted a decrease in respiratory frequency ( < 0.001, r = 0.61; < 0.001, r = 0.61, respectively), and an increase in peripheral oxygen saturation ( < 0.001, r = 0.50; < 0.001, r = 0.46, respectively), along with blood pressure decreases in all mean values, and a significant decrease in systolic pressure, considering all conditions ( = 0.034, W = 0.08). There were significant increases in standard deviation of HR during abdominal and complete yoga breathing techniques compared with the baseline ( = 0.003, r = 0.31; < 0.001, r = 0.47, respectively), indicating enhanced parasympathetic activity. Moreover, the complete breathing technique exhibited the greatest variability in HRV measures, with several significant differences compared with abdominal breathing (standard deviation of HR, < 0.001, r = 0.42; SD2, standard deviation of points perpendicular to the Poincaré parallel line, < 0.003, r = 0.31; SD1/SD2, < 0.003, r = 0.31), suggesting a more profound impact on autonomic modulation. simple, inexpensive and non-intrusive abdominal and complete yoga breathing techniques can effectively and momentarily enhance HRV and oxygen saturation in adults, mature adults and the elderly.