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[综合性本科实验:磁性固相萃取-高效液相色谱法测定茶叶中的苯脲类除草剂]

[Comprehensive undergraduate experiment: determination of phenylurea herbicide in tea by magnetic solid-phase extraction-high performance liquid chromatography].

作者信息

Liu Zhuang, Su Ying, Yang Dong-Wei, Li Li-Mei, Xu Xu

机构信息

College of Science, Shenyang Agricultural University, Shenyang 110866, China.

College of Chemistry, Liaoning University, Shenyang 110036, China.

出版信息

Se Pu. 2024 Nov;42(11):1094-1100. doi: 10.3724/SP.J.1123.2024.04035.

DOI:10.3724/SP.J.1123.2024.04035
PMID:39449517
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11519765/
Abstract

Against the backdrop of the Ministry of Education's promotion of new agricultural science construction and interdisciplinary integration, a comprehensive chemistry experiment to enhance the practical skills of students in preparing biomass functional materials and detecting pesticide residues was designed. Natural loofah was utilized as a precursor in synthesizing nitrogen-doped magnetic porous carbon materials, which were then applied in a magnetic solid-phase extraction (MSPE) technique. Subsequently, high performance liquid chromatography (HPLC) was employed to analyze and detect the phenylurea herbicide monuron in tea. The experimental process included material synthesis, characterization, optimization of the MSPE conditions, adsorption performance studies, and HPLC, reflecting its scientific, systematic nature and providing a comprehensive learning platform for students. It not only deepens student understanding of the relationship between material characterization and application, but also improves their experimental design and problem-solving capacities. Moreover, by integrating cutting-edge science, ideological and political education, and experimental training, it not only stimulates student interest in scientific research and cultivates innovative thinking and practical skills, but also strengthens their feelings of social responsibility and historical mission. This approach realizes the comprehensive educational goals of experimental training and lays the foundation for nurturing high-quality talent with a global perspective and sense of social responsibility.

摘要

在教育部推进新农科建设和学科交叉融合的背景下,设计了一个综合化学实验,以提高学生制备生物质功能材料和检测农药残留的实践技能。天然丝瓜被用作合成氮掺杂磁性多孔碳材料的前驱体,然后将其应用于磁固相萃取(MSPE)技术。随后,采用高效液相色谱(HPLC)对茶叶中的苯基脲类除草剂灭草隆进行分析检测。实验过程包括材料合成、表征、MSPE条件优化、吸附性能研究以及HPLC分析,体现了实验的科学性、系统性,为学生提供了一个综合性学习平台。它不仅加深了学生对材料表征与应用之间关系的理解,还提高了他们的实验设计和解决问题的能力。此外,通过融合前沿科学、思想政治教育和实验训练,不仅激发了学生对科研的兴趣,培养了创新思维和实践技能,还强化了他们的社会责任感和历史使命感。这种方式实现了实验训练的综合教育目标,为培养具有全球视野和社会责任感的高素质人才奠定了基础。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2a58/11519765/4eb49f504d16/cjc-42-11-1094-img_7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2a58/11519765/96359b068287/cjc-42-11-1094-img_1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2a58/11519765/46204abd24c1/cjc-42-11-1094-img_2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2a58/11519765/7d00ce100fe4/cjc-42-11-1094-img_3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2a58/11519765/44dcdf3ce58b/cjc-42-11-1094-img_4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2a58/11519765/84ef7416fbf2/cjc-42-11-1094-img_5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2a58/11519765/88698e33af47/cjc-42-11-1094-img_6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2a58/11519765/4eb49f504d16/cjc-42-11-1094-img_7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2a58/11519765/96359b068287/cjc-42-11-1094-img_1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2a58/11519765/46204abd24c1/cjc-42-11-1094-img_2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2a58/11519765/7d00ce100fe4/cjc-42-11-1094-img_3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2a58/11519765/44dcdf3ce58b/cjc-42-11-1094-img_4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2a58/11519765/84ef7416fbf2/cjc-42-11-1094-img_5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2a58/11519765/88698e33af47/cjc-42-11-1094-img_6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2a58/11519765/4eb49f504d16/cjc-42-11-1094-img_7.jpg

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本文引用的文献

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[Comprehensive undergraduate experiment: synthesis and chromism of 4-[bis(4-methylphenyl)amino] benzaldehyde].[综合性本科实验:4-[双(4-甲基苯基)氨基]苯甲醛的合成与变色现象]
Se Pu. 2024 Mar 8;42(3):304-308. doi: 10.3724/SP.J.1123.2023.11009.
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Facile synthesis of hydroxylated triazine-based magnetic microporous organic network for ultrahigh adsorption of phenylurea herbicides: An experimental and density-functional theory study.用于苯基脲类除草剂超高吸附的羟基化三嗪基磁性微孔有机网络的简便合成:一项实验与密度泛函理论研究
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Nanoconfinement of functionalized ionic liquid for enhanced adsorption and rapid sensitive detection of phenylurea herbicides in food and environmental samples.
纳米限域功能化离子液体用于增强食品和环境样品中苯脲类除草剂的吸附和快速灵敏检测。
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[Sulfonated magnetic graphite carbon nitride solid-phase extraction-ultra performance liquid chromatography-tandem mass spectrometry for screening malachite green and leucomalachite green in freshwater fish].磺化磁性石墨相氮化碳固相萃取-超高效液相色谱-串联质谱法用于筛查淡水鱼中的孔雀石绿和隐色孔雀石绿
Se Pu. 2023 Aug;41(8):673-682. doi: 10.3724/SP.J.1123.2022.12009.
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[Polyamidoamine dendrimer-functionalized silica nanocomposite with polydopamine coating for dispersive micro solid-phase extraction of benzoylurea insecticides in water samples].[聚酰胺-胺树枝状大分子功能化二氧化硅纳米复合材料及其聚多巴胺涂层用于水样中苯甲酰脲类杀虫剂的分散微固相萃取]
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