Department of Microbiology, Key Lab of Microbiology for Agricultural Environment, Ministry of Agriculture, College of Life Sciences , Nanjing Agricultural University , 210095 Nanjing , China.
Jiangsu Provincial Key Lab for Organic Solid Waste Utilization , Nanjing Agricultural University , Nanjing 210095 , China.
J Agric Food Chem. 2018 Nov 28;66(47):12479-12489. doi: 10.1021/acs.jafc.8b03703. Epub 2018 Nov 13.
Phenylurea herbicides (PHs) are frequently detected as major water contaminants in areas where there is extensive use. In this study, Diaphorobacter sp. strain LR2014-1, which initially hydrolyzes linuron to 3,4-dichloroanaline, and Achromobacter sp. strain ANB-1, which further mineralizes the produced aniline derivatives, were isolated from a linuron-mineralizing consortium despite being present at low abundance in the community. The synergistic catabolism of linuron by the consortium containing these two strains resulted in more efficient catabolism of linuron and growth of both strains. Strain LR2014-1 harbors two evolutionary divergent hydrolases from the amidohydrolase superfamily Phh and the amidase superfamily TccA2, which functioned complementarily in the hydrolysis of various types of PHs, including linuron ( N-methoxy- N-methyl-substituted), diuron, chlorotoluron, fluomethuron ( N, N-dimethyl-substituted), and siduron. These findings show that a bacterial consortium can contain catabolically synergistic species for PH mineralization, and one strain could harbor functionally complementary hydrolases for a broadened substrate range.
苯脲类除草剂（PHs）在广泛使用的地区经常被检测为主要的水污染物。在这项研究中，尽管在群落中的丰度较低，但仍从一个能够将利谷隆矿化的联合体中分离出了 Diaphorobacter sp. strain LR2014-1 和 Achromobacter sp. strain ANB-1，前者最初将利谷隆水解为 3,4-二氯苯胺，后者进一步矿化生成的苯胺衍生物。尽管这两种菌株在群落中丰度较低，但它们所组成的联合体能够协同代谢利谷隆，从而更有效地代谢利谷隆并促进这两种菌株的生长。菌株 LR2014-1 含有两种来自酰胺水解酶超家族 Phh 和酰胺酶超家族 TccA2 的进化上不同的水解酶，它们在水解各种类型的 PHs（包括 N-甲氧基-N-甲基取代的利谷隆、敌草隆、绿麦隆、氟乐灵（N,N-二甲基取代的）和噻唑隆）方面具有互补功能。这些发现表明，细菌联合体可以包含对 PH 矿化具有代谢协同作用的物种，而一种菌株可以拥有功能互补的水解酶，以扩大其底物范围。
