Division of Applied Life Science (BK21 Four), Gyeongsang National University, Jinju 52828, Republic of Korea.
Institute of Agriculture & Life Science, Gyeongsang National University, Jinju 52828, Republic of Korea.
Cells. 2024 Oct 19;13(20):1734. doi: 10.3390/cells13201734.
This study was conducted to analyze the viability of primary chicken embryo fibroblasts and the efficiency of adipogenic trans-differentiation for cultured meat production. In isolating chicken embryo fibroblasts (CEFs) from a heterogeneous cell pool containing chicken satellite cells (CSCs), over 90% of CEFs expressed CD29 and vimentin. The analysis of the proliferative capabilities of CEFs revealed no significant differences in EdU-positive cells (%), cumulative cell number, doubling time, and growth rate from passage 1 to passage 9 ( > 0.05). This indicates that CEFs can be isolated by 2 h of pre-plating and survive stably up to passage 9, and that primary fibroblasts can serve as a valuable cell source for the cultured meat industry. Adipogenic trans-differentiation was induced up to passage 9 of CEFs. As passages increased, lipid accumulation and adipocyte size significantly decreased ( < 0.05). The reduced differentiation rate of primary CEFs with increasing passages poses a major challenge to the cost and efficiency of cultured meat production. Thus, effective cell management and the maintenance of cellular characteristics for a long time are crucial for ensuring stable and efficient cultured fat production in the cultured meat industry.
本研究旨在分析原代鸡胚成纤维细胞的活力和脂肪生成转分化效率，以用于培养肉生产。在从含有鸡卫星细胞（CSCs）的异质细胞群中分离鸡胚成纤维细胞（CEFs）时，超过 90%的 CEFs 表达 CD29 和波形蛋白。CEFs 增殖能力的分析表明，EdU 阳性细胞（%）、累积细胞数、倍增时间和从第 1 代到第 9 代的生长速率没有显著差异（>0.05）。这表明可以通过 2 小时的预铺板分离 CEFs，并且在第 9 代之前稳定存活，原代成纤维细胞可以作为培养肉行业有价值的细胞来源。CEFs 可诱导至第 9 代进行脂肪生成转分化。随着传代次数的增加，脂质积累和脂肪细胞大小显著减少（<0.05）。随着传代次数的增加，原代 CEFs 的分化率降低，这对培养肉生产的成本和效率构成了重大挑战。因此，有效的细胞管理和长时间维持细胞特性对于确保培养肉行业中稳定高效的培养脂肪生产至关重要。
