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一种测量耵聍产生速率的技术。

A technique for measuring the rate of cerumen production.

作者信息

Cipriani C, Taborelli G, Cardo P P, Rebora A

出版信息

Laryngoscope. 1986 Feb;96(2):204-5. doi: 10.1288/00005537-198602000-00015.

DOI:10.1288/00005537-198602000-00015
PMID:3945151
Abstract

A technique is described for quantitatively measuring the rate of cerumen production. Two different washing solutions were tried. A mixture of alcohol/ether (3:1 v/v) appeared to be the best extracting solution yielding 2.05 +/- 0.22 versus 0.73 +/- 0.14 mg/week per ear obtained with Triton X-100 solution. In addition, reproducibility was definitely better. Triton X-100 may be useful whenever microbiologic evaluations are requested. Total lipids accounted for 73.3% of cerumen extracted with the former solution, triglycerides being 19.5%, and cholesterol 7.2%. Our extracting technique appears to be a good estimate of the cerumen global output and may supply important information about cerumen in several biologic conditions.

摘要

本文描述了一种定量测量耵聍产生速率的技术。尝试了两种不同的清洗溶液。酒精/乙醚(3:1 v/v)混合物似乎是最佳提取溶液,每只耳朵每周产生2.05±0.22毫克,而用Triton X-100溶液获得的量为0.73±0.14毫克/周。此外,重现性肯定更好。每当需要进行微生物学评估时,Triton X-100可能会有用。用前一种溶液提取的耵聍中总脂质占73.3%,甘油三酯占19.5%,胆固醇占7.2%。我们的提取技术似乎能很好地估计耵聍的总体产量,并可能提供有关几种生物学条件下耵聍的重要信息。

相似文献

1
A technique for measuring the rate of cerumen production.一种测量耵聍产生速率的技术。
Laryngoscope. 1986 Feb;96(2):204-5. doi: 10.1288/00005537-198602000-00015.
2
Production rate and composition of cerumen: influence of sex and season.耵聍的产生速率和成分:性别与季节的影响
Laryngoscope. 1990 Mar;100(3):275-6. doi: 10.1288/00005537-199003000-00012.
3
The comparison of lipids between dry and wet types of cerumen.干性与湿性耵聍的脂质比较。
Tohoku J Exp Med. 1967 Mar;91(3):227-37. doi: 10.1620/tjem.91.227.
4
[The effects of Triton WR 1339 on the secretion of bile lipids in the rat].[曲拉通WR 1339对大鼠胆汁脂质分泌的影响]
Biomedicine. 1976 Jul;25(6):228-31.
5
Composition of cerumen lipids.耵聍脂质的组成。
J Am Acad Dermatol. 1990 Nov;23(5 Pt 1):845-9. doi: 10.1016/0190-9622(90)70301-w.
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Human ceruminous gland innervation.人外耳道耵聍腺的神经支配。
J Laryngol Otol. 1981 Jan;95(1):11-5. doi: 10.1017/s0022215100090344.
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Enzymatic determination of triglyceride, free cholesterol, and total cholesterol in tissue lipid extracts.组织脂质提取物中甘油三酯、游离胆固醇和总胆固醇的酶法测定。
Clin Biochem. 1993 Feb;26(1):39-42. doi: 10.1016/0009-9120(93)90015-x.
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Studies on the chemical composition of human normal cerumen. 4. Separation of lipid fraction by silicic acid column chromatography and fatty acid composition.人正常耵聍的化学成分研究。4. 硅胶柱色谱法分离脂质部分及脂肪酸组成
Sapporo Igaku Zasshi. 1965 Oct-Nov;28(4):315-9.
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Investigation of age, sex and menstrual stage variation in human cerumen lipid composition by high performance thin layer chromatography.通过高效薄层色谱法研究人耳垢脂质成分的年龄、性别和月经阶段差异。
J Laryngol Otol. 2008 Sep;122(9):881-6. doi: 10.1017/S0022215107000783. Epub 2007 Oct 12.
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[Geographic variation and biological analysis of ear wax, with special reference to biochemical and immunochemical specificity of the dry and wet ear wax].耳垢的地理变异与生物学分析,特别提及干性和湿性耳垢的生化及免疫化学特异性
J Dent Educ. 1969 Dec;33(4):552-3.

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Ear wax: a new biological monitoring medium for metals?耳垢:一种用于金属检测的新型生物监测介质?
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