Prodan-Bărbulescu Cătălin, Ghenciu Laura Andreea, Şeclăman Edward, Bujor Georgeta Cristiana, Enătescu Virgil, Danila Alexandra-Ioana, Dăescu Ecaterina, Rosu Luminioara Maria, Faur Ionuţ Flaviu, Tuţac Paul, Varga Norberth-Istvan, Sonia Tanasescu, Duță Ciprian
Faculty of Medicine, "Victor Babeş" University of Medicine and Pharmacy Timisoara, 2nd Eftimie Murgu Square, 300041 Timisoara, Romania.
Department I-Discipline of Anatomy and Embryology, Faculty of Medicine, "Victor Babeş" University of Medicine and Pharmacy Timisoara, 2nd Eftimie Murgu Square, 300041 Timișoara, Romania.
Curr Issues Mol Biol. 2024 Sep 27;46(10):10846-10853. doi: 10.3390/cimb46100644.
Major depressive disorder (MDD) is a complex mental health condition with a multifaceted and incompletely elucidated pathophysiology. MicroRNAs (miRNAs) have emerged as potential biomarkers due to their role in gene regulation and the observed dysregulation in MDD. The aim of this study is to detect the presence of specific molecular diagnostic biomarkers in major depressive disorder. This cross-sectional study analyzed plasma miRNA expression in ten MDD patients and eight healthy controls using real-time PCR. Differentially expressed miRNAs were identified using independent -tests, and their diagnostic potential was assessed with ROC curve analysis. Fifteen miRNAs exhibited significant dysregulation in MDD patients. Notably, hsa-miR-29c-3p, hsa-miR-376a-3p, hsa-miR-532-5p, and hsa-miR-339-5p showed excellent discriminatory power (AUC > 0.8). This study identifies differentially expressed plasma miRNAs in MDD, suggesting their potential for improved diagnosis and personalized treatment. However, further validation in larger cohorts and investigation into their functional roles are warranted.
重度抑郁症（MDD）是一种复杂的心理健康状况，其病理生理学多方面且尚未完全阐明。微小RNA（miRNA）因其在基因调控中的作用以及在MDD中观察到的失调现象而成为潜在的生物标志物。本研究的目的是检测重度抑郁症中特定分子诊断生物标志物的存在。这项横断面研究使用实时聚合酶链反应分析了10名MDD患者和8名健康对照者血浆中的miRNA表达。使用独立样本t检验鉴定差异表达的miRNA，并通过ROC曲线分析评估其诊断潜力。15种miRNA在MDD患者中表现出显著失调。值得注意的是，hsa-miR-29c-3p、hsa-miR-376a-3p、hsa-miR-532-5p和hsa-miR-339-5p显示出优异的鉴别能力（曲线下面积>0.8）。本研究鉴定了MDD中差异表达的血浆miRNA，表明它们在改善诊断和个性化治疗方面的潜力。然而，需要在更大的队列中进一步验证并研究它们的功能作用。
