Lv Tao, Jiang Li, Kong Lingxiang, Yang Jiayin
Department of Hepato‑Biliary‑Pancreatic Surgery, West China Hospital of Sichuan University, Chengdu, Sichuan 610041, P.R. China.
Oncol Rep. 2020 Mar;43(3):953-964. doi: 10.3892/or.2020.7469. Epub 2020 Jan 17.
Aberrant expression of microRNAs (miRNAs) has been widely reported in many malignant tumors, and dysregulated miRNAs play an important role in the malignant progression of tumors. It has been reported that miR‑29c‑3p expression is dysregulated in tumors and promotes the development of tumors, especially in hepatocellular carcinoma (HCC). However, the specific mechanism of miR‑29c‑3p in HCC is not clear. The present study demonstrated that miR‑29c‑3p was expressed at low levels in HCC patients and cell lines and that its decreased expression was closely related to poor prognosis of HCC patients. Overexpression of miR‑29c‑3p could significantly inhibit the proliferation and migration of HCC cells in vitro and suppress the HCC tumor growth in vivo. The luciferase reporter assay demonstrated that miR‑29c‑3p directly bound to tripartite motif containing 31 (TRIM31) and suppressed TRIM31 expression. Finally, upregulation of TRIM31 could partially abolish the tumor suppressing roles of miR‑29c‑3p in HCC. Overall, miR‑29c‑3p, as a tumor suppressor gene, was revealed to inhibit the malignant progression of HCC by reducing the expression of TRIM31 and may be used as a potential therapeutic target for the precise treatment of HCC.
微小RNA（miRNA）的异常表达在许多恶性肿瘤中已被广泛报道，并且失调的miRNA在肿瘤的恶性进展中起重要作用。据报道，miR-29c-3p在肿瘤中表达失调并促进肿瘤发展，尤其是在肝细胞癌（HCC）中。然而，miR-29c-3p在HCC中的具体机制尚不清楚。本研究表明，miR-29c-3p在HCC患者和细胞系中低表达，其表达降低与HCC患者的不良预后密切相关。miR-29c-3p的过表达可显著抑制体外HCC细胞的增殖和迁移，并抑制体内HCC肿瘤生长。荧光素酶报告基因检测表明，miR-29c-3p直接与含三联基序蛋白31（TRIM31）结合并抑制TRIM31表达。最后，TRIM31的上调可部分消除miR-29c-3p在HCC中的抑癌作用。总体而言，miR-29c-3p作为一种抑癌基因，通过降低TRIM31的表达来抑制HCC的恶性进展，可能作为HCC精准治疗的潜在靶点。
