• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

两种用于多重检测蚊子中登革病毒1-4型和基孔肯雅病毒的新型逆转录聚合酶链反应（RT-PCR）和逆转录定量聚合酶链反应（RT-qPCR）方法的潜在性能

Potential Performance of Two New RT-PCR and RT-qPCR Methods for Multiplex Detection of Dengue Virus Serotypes 1-4 and Chikungunya Virus in Mosquitoes.

作者信息

Gomgnimbou Michel Kiréopori, Belem Louis Robert Wendyam, Bilgo Etienne, Amara Miriam Félicité, Laouali Zouera, Ouari Ali, Bayala Toussaint, Gnada Kobo, Yao Raymond Kharlis, Namountougou Moussa, Sangaré Ibrahim

机构信息

Centre d'Excellence Africain en Innovations Biotechnologiques pour l'Elimination des Maladies à Transmission Vectorielle (CEA/ITECH-MTV), Université Nazi BONI, Bobo-Dioulasso 01 BP 1091, Burkina Faso.

Laboratoire de Recherche, Centre MURAZ, Institut National de Santé Publique, Bobo-Dioulasso BP 10278, Burkina Faso.

出版信息

Curr Issues Mol Biol. 2024 Sep 30;46(10):11048-11056. doi: 10.3390/cimb46100656.

DOI:10.3390/cimb46100656
PMID:39451536
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11505945/
Abstract

Mosquitoes of the genus are the most important arthropod disease vector. Dengue virus (DENV) and Chikungunya virus (CHIKV) are the main arboviruses distributed throughout the world. Based on entomo-virological surveillance, appropriate public health strategies can be adopted to contain cases and control outbreaks. This study aims to show the potential performance of two new molecular methods for detecting DENV serotypes and CHIKV in mosquitoes. Mosquitoes were collected in urban and sylvatic areas of Bobo-Dioulasso, Burkina Faso, between July and August 2023. DENV and CHIKV were screened using new multiplex RT-PCR and RT-qPCR methods. A total of 2150 mosquitoes were trapped, consisting of 976 Aedes (959 , 6 , and 11 ) and 1174 sp. These were grouped into 39 pools, with each pool containing a maximum of 30 mosquitoes. Molecular screening revealed that 7.7% (3/39) of the pools were positive for DENV. Specifically, DENV-1 was detected in one pool (1/3), and DENV-3 was found in two pools (2/3). All pools tested negative for CHIKV. The overall minimum infection rate (MIR) of DENV in this study was 3.07 (95% CI: 2.24-19.86). This study shows the usefulness of our new molecular tools for the surveillance of DENV serotypes and CHIKV.

摘要

属的蚊子是最重要的节肢动物疾病传播媒介。登革病毒（DENV）和基孔肯雅病毒（CHIKV）是分布于全球的主要虫媒病毒。基于昆虫病毒学监测，可采用适当的公共卫生策略来控制病例和疫情暴发。本研究旨在展示两种检测蚊子中DENV血清型和CHIKV的新分子方法的潜在性能。2023年7月至8月期间，在布基纳法索博博迪乌拉索的城市和森林地区采集了蚊子。使用新的多重逆转录聚合酶链反应（RT-PCR）和逆转录定量聚合酶链反应（RT-qPCR）方法对DENV和CHIKV进行筛查。共捕获2150只蚊子，其中包括976只伊蚊（959只埃及伊蚊、6只白纹伊蚊和11只其他伊蚊）和1174只其他种类蚊子。这些蚊子被分成39组，每组最多包含30只蚊子。分子筛查显示，7.7%（3/39）的组DENV呈阳性。具体而言，在一组（1/3）中检测到DENV-1，在两组（2/3）中发现DENV-3。所有组CHIKV检测均为阴性。本研究中DENV的总体最低感染率（MIR）为3.07（95%置信区间：2.24 - 19.86）。本研究表明了我们新的分子工具在监测DENV血清型和CHIKV方面的有用性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f485/11505945/6ef0d3c5d545/cimb-46-00656-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f485/11505945/b0c4ae5a72cb/cimb-46-00656-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f485/11505945/23fca238157f/cimb-46-00656-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f485/11505945/6ef0d3c5d545/cimb-46-00656-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f485/11505945/b0c4ae5a72cb/cimb-46-00656-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f485/11505945/23fca238157f/cimb-46-00656-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f485/11505945/6ef0d3c5d545/cimb-46-00656-g003.jpg

相似文献

1
Potential Performance of Two New RT-PCR and RT-qPCR Methods for Multiplex Detection of Dengue Virus Serotypes 1-4 and Chikungunya Virus in Mosquitoes.两种用于多重检测蚊子中登革病毒1-4型和基孔肯雅病毒的新型逆转录聚合酶链反应（RT-PCR）和逆转录定量聚合酶链反应（RT-qPCR）方法的潜在性能
Curr Issues Mol Biol. 2024 Sep 30;46(10):11048-11056. doi: 10.3390/cimb46100656.
2
Utilization of novel molecular multiplex methods for the detection and, epidemiological surveillance of dengue virus serotypes and chikungunya virus in Burkina Faso, West Africa.利用新型分子多重方法检测和进行西非法属布基纳法索登革热病毒血清型和基孔肯雅病毒的流行病学监测。
Mol Biol Rep. 2024 Aug 14;51(1):906. doi: 10.1007/s11033-024-09847-1.
3
Entomo-virological surveillance strategy for dengue, Zika and chikungunya arboviruses in field-caught Aedes mosquitoes in an endemic urban area of the Northeast of Brazil.巴西东北部流行地区野外捕获的埃及伊蚊中登革热、寨卡和基孔肯雅热虫媒病毒的 Entomovirological 监测策略。
Acta Trop. 2019 Sep;197:105061. doi: 10.1016/j.actatropica.2019.105061. Epub 2019 Jun 10.
4
Isolation and molecular detection of dengue and chikungunya virus from field-collected adult mosquitoes in Kelantan, Malaysia.从马来西亚吉兰丹野外采集的成蚊中分离和分子检测登革热和基孔肯雅病毒。
J Vector Borne Dis. 2024 Jan 1;61(1):61-71. doi: 10.4103/0972-9062.392269. Epub 2024 Mar 23.
5
Molecular detection of dengue and chikungunya viruses in surveillance of wild-caught Aedes mosquitoes in Punjab, North India.在印度北部旁遮普邦对野生捕获的伊蚊进行监测时，检测到登革热和基孔肯雅热病毒。
Trans R Soc Trop Med Hyg. 2024 Feb 1;118(2):95-101. doi: 10.1093/trstmh/trad054.
6
Development of Multiplex Molecular Assays for Simultaneous Detection of Dengue Serotypes and Chikungunya Virus for Arbovirus Surveillance.用于虫媒病毒监测的同时检测登革热血清型和基孔肯雅病毒的多重分子检测方法的开发
Curr Issues Mol Biol. 2024 Mar 6;46(3):2093-2104. doi: 10.3390/cimb46030134.
7
Surveillance of dengue virus in individual Aedes aegypti mosquitoes collected concurrently with suspected human cases in Tarlac City, Philippines.菲律宾塔拉克市采集的与疑似人类病例同时发生的埃及伊蚊个体中登革热病毒的监测。
Parasit Vectors. 2020 Nov 25;13(1):594. doi: 10.1186/s13071-020-04470-y.
8
Entomo-virological surveillance followed by serological active survey of symptomatic individuals is helpful to identify hotspots of early arbovirus transmission.昆虫病毒学监测结合症状性个体的血清学主动调查有助于确定早期虫媒病毒传播的热点。
Front Public Health. 2022 Oct 28;10:1024187. doi: 10.3389/fpubh.2022.1024187. eCollection 2022.
9
Dengue serotype circulation in natural populations of Aedes aegypti.埃及伊蚊自然种群中的登革热血清型传播情况。
Acta Trop. 2017 Dec;176:140-143. doi: 10.1016/j.actatropica.2017.07.014. Epub 2017 Jul 23.
10
A Novel Multiplex RT-PCR Assay for Simultaneous Detection of Dengue and Chikungunya Viruses.一种用于同时检测登革热病毒和基孔肯雅病毒的新型多重逆转录聚合酶链反应检测方法。
Int J Mol Sci. 2020 Nov 5;21(21):8281. doi: 10.3390/ijms21218281.

引用本文的文献

1
Single-cell pseudotime and intercellular communication analysis reveals heterogeneity and immune microenvironment in oral cancer.单细胞伪时间和细胞间通讯分析揭示口腔癌中的异质性和免疫微环境。
Discov Oncol. 2025 Feb 10;16(1):151. doi: 10.1007/s12672-025-01918-4.

本文引用的文献

1
Development of Multiplex Molecular Assays for Simultaneous Detection of Dengue Serotypes and Chikungunya Virus for Arbovirus Surveillance.用于虫媒病毒监测的同时检测登革热血清型和基孔肯雅病毒的多重分子检测方法的开发
Curr Issues Mol Biol. 2024 Mar 6;46(3):2093-2104. doi: 10.3390/cimb46030134.
2
Molecular surveillance of dengue virus in field-collected mosquitoes from Bhopal, central India: evidence of circulation of a new lineage of serotype 2.印度中部博帕尔地区野外采集蚊子中登革病毒的分子监测：2型新谱系传播的证据
Front Microbiol. 2023 Sep 14;14:1260812. doi: 10.3389/fmicb.2023.1260812. eCollection 2023.
3
Enhanced Arbovirus Surveillance with High-Throughput Metatranscriptomic Processing of Field-Collected Mosquitoes.
高通量宏转录组学处理野外采集的蚊子以增强虫媒病毒监测。
Viruses. 2022 Dec 11;14(12):2759. doi: 10.3390/v14122759.
4
Review of the ecology and behaviour of and in Western Africa and implications for vector control.西非[具体物种名称1]和[具体物种名称2]的生态学与行为综述及其对病媒控制的影响
Curr Res Parasitol Vector Borne Dis. 2022;2:100074. doi: 10.1016/j.crpvbd.2021.100074.
5
Seroepidemiological Reconstruction of Long-term Chikungunya Virus Circulation in Burkina Faso and Gabon.布基纳法索和加蓬的基孔肯雅病毒长期传播的血清流行病学重建。
J Infect Dis. 2023 Jan 11;227(2):261-267. doi: 10.1093/infdis/jiac246.
6
Detection of DENV-2 and Insect-Specific Flaviviruses in Mosquitoes Collected From Jeddah, Saudi Arabia.从沙特阿拉伯吉达采集的蚊子中检测到登革热病毒 2 型和昆虫特异性黄病毒。
Front Cell Infect Microbiol. 2021 Feb 25;11:626368. doi: 10.3389/fcimb.2021.626368. eCollection 2021.
7
A Novel Multiplex RT-PCR Assay for Simultaneous Detection of Dengue and Chikungunya Viruses.一种用于同时检测登革热病毒和基孔肯雅病毒的新型多重逆转录聚合酶链反应检测方法。
Int J Mol Sci. 2020 Nov 5;21(21):8281. doi: 10.3390/ijms21218281.
8
The epidemiology of dengue outbreaks in 2016 and 2017 in Ouagadougou, Burkina Faso.2016年和2017年布基纳法索瓦加杜古登革热疫情的流行病学情况。
Heliyon. 2020 Jul 14;6(7):e04389. doi: 10.1016/j.heliyon.2020.e04389. eCollection 2020 Jul.
9
Monitoring Insecticide Susceptibility in Populations from the Two Biggest Cities, Ouagadougou and Bobo-Dioulasso, in Burkina Faso: Implication of Metabolic Resistance.监测布基纳法索两个最大城市瓦加杜古和博博迪乌拉索的昆虫种群对杀虫剂的敏感性：代谢抗性的影响。
Trop Med Infect Dis. 2020 May 27;5(2):84. doi: 10.3390/tropicalmed5020084.
10
Risk of arbovirus emergence via bridge vectors: case study of the sylvatic mosquito Aedes malayensis in the Nakai district, Laos.通过桥梁媒介物出现虫媒病毒的风险：老挝那凯地区森林蚊子 Aedes malayensis 的案例研究。
Sci Rep. 2020 May 8;10(1):7750. doi: 10.1038/s41598-020-64696-9.