Jiang Xiaona, Tian Lijing, Ren Wanying, Li Chitao, Hu Xuesong, Ge Yanlong, Cheng Lei, Shi Xiaodan, Jia Zhiying
Heilongjiang River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Harbin 150076, China.
Key Laboratory of Freshwater Aquatic Biotechnology and Breeding, Ministry of Agriculture and Rural Affairs, Harbin 150076, China.
Curr Issues Mol Biol. 2024 Oct 21;46(10):11714-11728. doi: 10.3390/cimb46100696.
Phosphoinositide 3-kinases (PI3Ks) are a class of key regulatory factors in eukaryotes that can inhibit viral replication by influencing autophagy. Currently, cyprinid herpesvirus 3 (CyHV-3) poses a serious threat to common carp culture. However, PI3K has not yet been identified in common carp. In this study, full-length from common carp (), consisting of an open reading frame (ORF) of 2664 bp encoding a polypeptide of 887 amino acids, with a predicted molecular mass of 101.19 kDa and a theoretical isoelectric point (pI) of 5.97, was cloned. The amino acid and nucleotide sequences of CcPI3KC3 displayed high similarity to yellow catfish's () PI3KC3. The tissue expression profile revealed that the mRNA levels of in the liver, spleen, and head kidney were significantly greater than those in the brain, heart, intestines, gills, eyes, testes, and ovaries of common carp. We compared the expression patterns of between "Longke-11" mirror carp (CyHV-3-resistant carp) and German mirror carp (non-resistant to CyHV-3) at different times (0, 48, 96, 144 h, 192, 240, 288 h post-infection (hpi)) after CyHV-3 infection. The results revealed that mRNA expression significantly increased in the early infection stage. In the CyHV-3-resistant mirror carp variety, the relative expression of was significantly greater at 48, 96, and 144 hpi compared with the nonbreeding strain groups after infection ( < 0.001). These results indicate that the full-length sequence was successfully cloned from common carp for the first time, and it might play an important role in the immune system of common carp against CyHV-3 infection. This study provides a theoretical basis for the molecular mechanism of CyHV-3 resistance.
磷脂酰肌醇3激酶（PI3Ks）是真核生物中的一类关键调控因子，可通过影响自噬来抑制病毒复制。目前，鲤疱疹病毒3型（CyHV-3）对鲤鱼养殖构成严重威胁。然而，鲤鱼中尚未鉴定出PI3K。在本研究中，克隆了鲤鱼（）的全长，其开放阅读框（ORF）为2664 bp，编码一个由887个氨基酸组成的多肽，预测分子量为101.19 kDa，理论等电点（pI）为5.97。CcPI3KC3的氨基酸和核苷酸序列与黄颡鱼（）的PI3KC3具有高度相似性。组织表达谱显示，在鲤鱼的肝脏、脾脏和头肾中，的mRNA水平显著高于大脑、心脏、肠道、鳃、眼睛、睾丸和卵巢中的水平。我们比较了“龙科11号”镜鲤（抗CyHV-3鲤鱼）和德国镜鲤（对CyHV-3不耐药）在CyHV-3感染后不同时间点（感染后0、48、96、144 h、192、240、288 h，hpi）的表达模式。结果显示，在感染早期，mRNA表达显著增加。在抗CyHV-3的镜鲤品种中，与感染后的非选育品系组相比，在48、96和144 hpi时的相对表达显著更高（<0.001）。这些结果表明，首次成功从鲤鱼中克隆出全长序列，它可能在鲤鱼抵抗CyHV-3感染的免疫系统中发挥重要作用。本研究为CyHV-3抗性的分子机制提供了理论依据。
