Puente-Marin Sara, Havarinasab Said
Division of Inflammation and Infection (II), Department of Biomedical and Clinical Sciences (BKV), Linköping University, 581 83 Linköping, Sweden.
Division of Clinical Chemistry and Pharmacology (KKF), Department of Biomedical and Clinical Sciences (BKV), Linköping University, 581 83 Linköping, Sweden.
Biology (Basel). 2024 Oct 11;13(10):812. doi: 10.3390/biology13100812.
To demonstrate causation or/and assess pathogenic mechanisms of environment-induced autoimmunity, various animal models that mimic the characteristics of the human autoimmune diseases need to be developed. Experimental studies in mice reveal the genetic factors that contribute to autoimmune diseases. Here, the immune response of two mouse strains congenic for non-H-2 genes, A.TL (H-2) and A.SW (H-2), was evaluated after 15 weeks' exposure to gold aurothiomalate (AuTM). AuTM-treated A.TL mice showed anti-nuclear antibodies (ANA) with homogenous and/or fine speckled staining patterns and serum autoantibodies to ds-DNA, chromatin, histones, and ribonucleoproteins (RNP). Female A.TL mice showed a stronger immune response than males, as well as an increase of B cells in their spleen after 15 weeks of gold exposure. A.SW exposed for AuTM showed the induction of anti-nucleolar antibodies (ANoA) with a clumpy staining pattern, as well as an increase in splenic B and T cells. The serum autoantibodies levels in A.SW mice were limited compared to those of A.TL mice. Overall, A.TL presents a stronger immune response after gold exposure than A.SW. The immune response developed in A.TL presents similarities with the clinical manifestations in human autoimmune diseases. Thus, gold-exposed A.TL could constitute a potential experimental mouse model for the study of autoimmunity.
为了证明环境诱导的自身免疫的因果关系和/或评估其致病机制，需要开发各种模拟人类自身免疫疾病特征的动物模型。对小鼠的实验研究揭示了导致自身免疫疾病的遗传因素。在此，对两种非H-2基因同基因的小鼠品系A.TL（H-2）和A.SW（H-2），在暴露于金硫代苹果酸（AuTM）15周后评估其免疫反应。经AuTM处理的A.TL小鼠显示出具有均匀和/或细颗粒状染色模式的抗核抗体（ANA）以及针对双链DNA、染色质、组蛋白和核糖核蛋白（RNP）的血清自身抗体。雌性A.TL小鼠比雄性表现出更强的免疫反应，并且在金暴露15周后其脾脏中的B细胞增加。暴露于AuTM的A.SW显示出具有块状染色模式的抗核仁抗体（ANoA）的诱导，以及脾脏B细胞和T细胞的增加。与A.TL小鼠相比，A.SW小鼠的血清自身抗体水平有限。总体而言，A.TL在金暴露后比A.SW表现出更强的免疫反应。A.TL中产生的免疫反应与人类自身免疫疾病的临床表现相似。因此，暴露于金的A.TL可能构成一种用于自身免疫研究的潜在实验小鼠模型。
