Liu Jia, Weng Qingqing, Da Dongxin, Yao Shuran, Zhang Ying, Wu Yang
Department of Preventive Dentistry, Shanghai Stomatological Hospital, School of Stomatology, Fudan University, Shanghai 200120, China.
Shanghai Key Laboratory of Craniomaxillofacial Development and Diseases, Fudan University, Shanghai 200120, China.
Antibiotics (Basel). 2024 Sep 30;13(10):930. doi: 10.3390/antibiotics13100930.
The oral cavity is an important but often overlooked reservoir for . The effective control and prevention of colonization and infection in the oral and maxillofacial regions are crucial for public health. Fluoride is widely used in dental care for its remineralization and antibacterial properties. However, its effectiveness against has not been thoroughly investigated. This study aimed to evaluate the potential of combining sodium fluoride (NaF) with compounds to enhance its antibacterial and antibiofilm effects against . We found that a urea derivative significantly enhances the efficacy of fluoride by promoting the retention of fluoride ions within the cells. The synergistic antibacterial and antibiofilm effects of BPU with NaF were confirmed through various assays, including checkerboard assays, time-kill assays, and growth curve analysis. These findings were further supported by additional methods, including transmission electron microscopy (TEM), in silico simulations, and gene overexpression studies. These findings suggest that targeting fluoride ion membrane exporters could enhance antibacterial efficacy. When combined with fluoride, 1,3-Bis [3,5-bis(trifluoromethyl)phenyl]urea (BPU) showed increased effectiveness in inhibiting growth and reducing established biofilms. This novel combination represents a promising therapeutic strategy for treating biofilm-associated infections, offering a new strategy in oral healthcare. To fully evaluate the clinical potential of this synergistic therapy, further in vivo studies are essential.
口腔是一个重要但常被忽视的[病原体名称]储存库。有效控制和预防口腔颌面部区域的[病原体名称]定植和感染对公共卫生至关重要。氟化物因其再矿化和抗菌特性而广泛应用于牙科护理。然而，其对[病原体名称]的有效性尚未得到充分研究。本研究旨在评估将氟化钠（NaF）与化合物结合以增强其对[病原体名称]的抗菌和抗生物膜作用的潜力。我们发现一种尿素衍生物通过促进氟离子在细胞内的保留显著提高了氟化物的功效。通过各种试验，包括棋盘试验、时间杀灭试验和生长曲线分析，证实了BPU与NaF的协同抗菌和抗生物膜作用。这些发现得到了包括透射电子显微镜（TEM）、计算机模拟和基因过表达研究等其他方法的进一步支持。这些发现表明，靶向氟离子膜转运蛋白可以提高抗菌功效。当与氟化物结合时，1,3-双[3,5-双（三氟甲基）苯基]脲（BPU）在抑制[病原体名称]生长和减少已形成的生物膜方面显示出更高的有效性。这种新型组合代表了一种治疗生物膜相关[病原体名称]感染的有前景的治疗策略，为口腔保健提供了一种新策略。为了全面评估这种协同治疗的临床潜力，进一步的体内研究至关重要。
