Lazić Vesna, Nedeljković Jovan M, Kokol Vanja
Vinča Institute of Nuclear Sciences-National Institute of the Republic of Serbia, University of Belgrade, Centre of Excellence for Photoconversion, 11000 Belgrade, Serbia.
Faculty of Mechanical Engineering, University of Maribor, 2000 Maribor, Slovenia.
J Funct Biomater. 2024 Oct 13;15(10):304. doi: 10.3390/jfb15100304.
Silver nanoparticles (Ag NPs) conjugated with amino-functionalized cellulose nanofibrils (NH-CNFs) were in situ-prepared by reducing silver ions with free amino groups from NH-CNFs. The spectroscopy and transmission electron microscopy measurements confirmed the presence of non-agglomerated nanometer-in-size Ag NPs within micrometer-large NH-CNFs of high (20 wt.-%) content. Although the consumption of amino groups during the formation of Ag NPs lowers the ζ-potential and surface charge of prepared inorganic-organic hybrids (from +31.3 to +19.9 mV and from 2.4 to 1.0 mmol/g at pH 7, respectively), their values are sufficiently positive to ensure electrostatic interaction with negatively charged cell walls of pathogens in acidic and slightly (up to pH ~8.5) alkaline solutions. The antimicrobial activity of hybrid microparticles against various pathogens (, , , and ) is comparable with pristine NH-CNFs. However, a long-timescale use of hybrids ensures the slow and controlled release of Ag ions to surrounding media (less than 1.0 wt.-% for one month).
通过用氨基功能化纤维素纳米纤丝（NH-CNFs）中的游离氨基还原银离子，原位制备了与氨基功能化纤维素纳米纤丝共轭的银纳米颗粒（Ag NPs）。光谱学和透射电子显微镜测量证实，在高含量（20重量%）的微米级NH-CNFs中存在未团聚的纳米级Ag NPs。尽管在Ag NPs形成过程中氨基的消耗降低了所制备的无机-有机杂化物的ζ电位和表面电荷（在pH值为7时，分别从+31.3 mV降至+19.9 mV，从2.4 mmol/g降至1.0 mmol/g），但它们的值仍具有足够的正电荷，以确保在酸性和微碱性（pH值高达约8.5）溶液中与带负电荷的病原体细胞壁发生静电相互作用。杂化微粒对各种病原体（、、和）的抗菌活性与原始NH-CNFs相当。然而，长期使用杂化物可确保Ag离子缓慢且可控地释放到周围介质中（一个月内释放量小于1.0重量%）。
