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人CD5和CD6清道夫受体串联肽作为潜在抗隐球菌药物的体外分析

In Vitro Analysis of Tandem Peptides from Human CD5 and CD6 Scavenger Receptors as Potential Anti-Cryptococcal Agents.

作者信息

Mourglia-Ettlin Gustavo, González-Porcile María Clara, Planells-Romeo Violeta, Long-Albín Antonella, Carrillo-Serradell Laura, Miles Sebastián, Lozano Francisco, Velasco-de-Andrés María

机构信息

Área Inmunología, Departamento de Biociencias (DEPBIO), Facultad de Química, Universidad de la República, Montevideo 11800, Uruguay.

Unidad Asociada de Inmunología, Instituto de Química Biológica (IQB), Facultad de Ciencias, Universidad de la República, Montevideo 11400, Uruguay.

出版信息

J Fungi (Basel). 2024 Sep 24;10(10):667. doi: 10.3390/jof10100667.

DOI:10.3390/jof10100667
PMID:39452619
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11508589/
Abstract

is included in the World Health Organization fungal priority pathogen list, complied to expedite improved research and public-health interventions. The limited number of available antifungal drugs, their associated toxicity, and the emergence of drug-resistant strains make the development of new therapeutic strategies mandatory. Pattern-recognition receptors (PRRs) from the host's innate immune system constitute a potential source of new antimicrobial agents. CD5 and CD6 are lymphoid members of the ancient scavenger receptor cysteine-rich superfamily (SRCR-SF) which bind pathogen-associated molecular patterns (PAMPs) of fungal and bacterial origin. Evidence supports the concept that such binding maps to 11-mer sequences present in each of their three SRCR extracellular domains. Herein, we have designed synthetic peptides containing tandems of such 11-mer sequences (namely CD5-T and CD6-T) and analyzed their -binding properties in vitro. Our results show both inhibitory effects on fungal growth and an ability to impact capsule formation and titanization, two critical virulence factors of involved in immune evasion. These effects hold promise for CD5-T and CD6-T peptides as single or adjuvant therapeutic agents against cryptococcosis.

摘要

被列入世界卫生组织真菌重点病原体清单，该清单旨在加快改进研究和公共卫生干预措施。可用抗真菌药物数量有限、其相关毒性以及耐药菌株的出现使得开发新的治疗策略成为必然。宿主先天免疫系统的模式识别受体（PRRs）构成了新抗菌剂的潜在来源。CD5和CD6是古老的富含半胱氨酸的清道夫受体超家族（SRCR-SF）的淋巴样成员，它们结合真菌和细菌来源的病原体相关分子模式（PAMPs）。有证据支持这样的概念，即这种结合映射到它们三个SRCR细胞外结构域中每个结构域存在的11聚体序列。在此，我们设计了包含此类11聚体序列串联的合成肽（即CD5-T和CD6-T），并在体外分析了它们的结合特性。我们的结果显示了对真菌生长的抑制作用以及影响荚膜形成和巨型化的能力，荚膜形成和巨型化是参与免疫逃避的两个关键毒力因子。这些作用使CD5-T和CD6-T肽有望作为抗隐球菌病的单一或辅助治疗剂。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e530/11508589/950042913ae5/jof-10-00667-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e530/11508589/7f63b1acbd4e/jof-10-00667-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e530/11508589/73139ce94f89/jof-10-00667-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e530/11508589/e42611688342/jof-10-00667-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e530/11508589/73a030191786/jof-10-00667-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e530/11508589/950042913ae5/jof-10-00667-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e530/11508589/7f63b1acbd4e/jof-10-00667-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e530/11508589/73139ce94f89/jof-10-00667-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e530/11508589/e42611688342/jof-10-00667-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e530/11508589/73a030191786/jof-10-00667-g004.jpg
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本文引用的文献

1
Global incidence and mortality of severe fungal disease.全球严重真菌感染的发病率和死亡率。
Lancet Infect Dis. 2024 Jul;24(7):e428-e438. doi: 10.1016/S1473-3099(23)00692-8. Epub 2024 Jan 12.
2
Antimicrobial peptides: An alternative to traditional antibiotics.抗菌肽：传统抗生素的替代品。
Eur J Med Chem. 2024 Feb 5;265:116072. doi: 10.1016/j.ejmech.2023.116072. Epub 2023 Dec 21.
3
Cryptococcal meningitis.隐球菌性脑膜炎
Nat Rev Dis Primers. 2023 Nov 9;9(1):62. doi: 10.1038/s41572-023-00472-z.
4
Antifungal Peptide SP1 Damages Polysaccharide Capsule of Cryptococcus neoformans and Enhances Phagocytosis of Macrophages.抗真菌肽SP1破坏新型隐球菌的多糖荚膜并增强巨噬细胞的吞噬作用。
Microbiol Spectr. 2023 Mar 14;11(2):e0456222. doi: 10.1128/spectrum.04562-22.
5
No Chance to Survive: -CBP-PepII Synthetic Peptide Acts on by Multiple Mechanisms of Action.无生存机会：-CBP-PepII合成肽通过多种作用机制发挥作用。
Antibiotics (Basel). 2023 Feb 12;12(2):378. doi: 10.3390/antibiotics12020378.
6
Anticryptococcal activity and mechanistic studies of short amphipathic peptides.短两性离子肽的抗隐球菌活性及作用机制研究
Arch Pharm (Weinheim). 2023 Apr;356(4):e2200576. doi: 10.1002/ardp.202200576. Epub 2023 Jan 2.
7
CD6 deficiency impairs early immune response to bacterial sepsis.CD6缺乏会损害对细菌性败血症的早期免疫反应。
iScience. 2022 Sep 5;25(10):105078. doi: 10.1016/j.isci.2022.105078. eCollection 2022 Oct 21.
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Treatment strategies for cryptococcal infection: challenges, advances and future outlook.隐球菌感染的治疗策略：挑战、进展与未来展望。
Nat Rev Microbiol. 2021 Jul;19(7):454-466. doi: 10.1038/s41579-021-00511-0. Epub 2021 Feb 8.
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