Wang Di, Zhang Haijue, Meng Lingkui, Tan Xinyu, Liu Rong, Gao Qingchao, Wu Yan, Zhu Yuhan, Ren Xueyan, Li Yongcai, Kong Qingjun
Xi'an Key Laboratory of Characteristic Fruit Storage and Preservation, Shaanxi Engineering Laboratory of Food Green Processing and Safety Control, College of Food Engineering and Nutritional Science, Shaanxi Normal University, Xi'an 710119, China.
College of Food Science and Engineering, Gansu Agricultural University, Lanzhou 730070, China.
J Fungi (Basel). 2024 Oct 19;10(10):726. doi: 10.3390/jof10100726.
Black spot caused by is one of the most common postharvest diseases in fruit and vegetables. A comprehensive investigation into its pathogenicity mechanism is imperative in order to propose a targeted and effective control strategy. The effect of nitric oxide (NO) on the pathogenicity of and its underlying mechanism was studied. The results showed that treatment with 0.5 mM L of sodium nitroprusside (SNP) (NO donor) increased the lesion diameter of in vivo and in vitro, which was 22.8% and 13.2% higher than that of the control, respectively. Exogenous NO treatment also induced endogenous NO accumulation by activating nitric oxide synthase (NOS). In addition, NO triggered an increase in reactive oxygen species (ROS) levels. NO enhanced activities and gene expression levels of NADPH oxidase (NOX), superoxide dismutase (SOD), catalase (CAT), ascorbate peroxidase (APX), glutathione peroxidase (GPX), and glutathione reductase (GR). Moreover, NO stimulated cell wall degrading enzymes by activating the corresponding gene expression in vivo and in vitro. These results suggested that exogenous NO promoted the pathogenicity of by inducing ROS accumulation and activating antioxidants and cell wall degrading enzymes. The present results could establish a theoretical foundation for the targeted control of the black spot disease in pear fruit.
由[病原菌名称缺失]引起的黑斑病是水果和蔬菜中最常见的采后病害之一。为了提出有针对性的有效防治策略，对其致病机理进行全面研究势在必行。研究了一氧化氮（NO）对[病原菌名称缺失]致病性的影响及其潜在机制。结果表明，用0.5 mM/L的硝普钠（SNP，NO供体）处理在体内和体外均增加了[病原菌名称缺失]的病斑直径，分别比对照高22.8%和13.2%。外源NO处理还通过激活一氧化氮合酶（NOS）诱导内源性NO积累。此外，NO引发了活性氧（ROS）水平的升高。NO增强了NADPH氧化酶（NOX）、超氧化物歧化酶（SOD）、过氧化氢酶（CAT）、抗坏血酸过氧化物酶（APX）、谷胱甘肽过氧化物酶（GPX）和谷胱甘肽还原酶（GR）的活性及基因表达水平。此外，NO在体内和体外通过激活相应基因表达刺激细胞壁降解酶。这些结果表明，外源NO通过诱导ROS积累以及激活抗氧化剂和细胞壁降解酶促进了[病原菌名称缺失]的致病性。本研究结果可为梨果实黑斑病的靶向防治奠定理论基础。
