Anne Saika, Friudenberg Alyssa D, Peterson Ryan L
Department of Biology, Texas State University, 601 University Drive, San Marcos, TX 78666, USA.
Department of Chemistry and Biochemistry, Texas State University, 601 University Drive, San Marcos, TX 78666, USA.
J Fungi (Basel). 2024 Oct 21;10(10):729. doi: 10.3390/jof10100729.
Copper is an essential micronutrient and the ability to scavenge tightly bound or trace levels of copper ions at the host-pathogen interface is vital for fungal proliferation in animal hosts. Recent studies suggest that trace metal ion acquisition is critical for the establishment and propagation of , the fungal pathogen responsible for white-nose syndrome (WNS), on their bat host. However, little is known about these metal acquisition pathways in In this study, we report the characterization of the high-affinity copper transporter VC83_00191 (CTR1a), which is implicated as a virulence factor associated with the WNS disease state. Using as a recombinant expression host, we find that CTR1a can efficiently traffic Cu ions into the yeast cytoplasm. Complementary studies in the native fungus provide evidence that CTR1a transcripts and protein levels are dictated by Cu-bioavailability in the growth media. Our study demonstrates that CTR1a is a functional high-affinity copper transporter and is relevant to Cu homeostasis pathways in
铜是一种必需的微量营养素，在宿主 - 病原体界面清除紧密结合或痕量水平的铜离子的能力对于真菌在动物宿主中的增殖至关重要。最近的研究表明，痕量金属离子的获取对于导致白鼻综合征（WNS）的真菌病原体在其蝙蝠宿主上的定殖和传播至关重要。然而，关于这些金属获取途径在 中知之甚少。在本研究中，我们报告了高亲和力铜转运蛋白VC83_00191（CTR1a）的特性，它被认为是与WNS疾病状态相关的毒力因子。使用 作为重组表达宿主，我们发现CTR1a可以有效地将铜离子转运到酵母细胞质中。在天然 真菌中的补充研究提供了证据，表明CTR1a转录本和蛋白质水平由生长培养基中的铜生物可利用性决定。我们的研究表明，CTR1a是一种功能性高亲和力铜转运蛋白，与 中的铜稳态途径相关。
