Spears Meghan, Cooper Gwendolyn, Sather Brett, Bailey Marguerite, Boles Jane A, Bothner Brian, Miles Mary P
Department of Food Systems, Nutrition, and Kinesiology, Montana State University, Bozeman, MT 59717, USA.
Department of Chemistry and Biochemistry, Montana State University, Bozeman, MT 59717, USA.
Metabolites. 2024 Oct 3;14(10):533. doi: 10.3390/metabo14100533.
BACKGROUND/OBJECTIVES: Cattle-feeding systems may have health implications for consumers of beef products. Organic grass-fed (GRA) and conventional (CON) cattle-feeding systems may result in beef products with differing metabolite profiles and therefore could impact the postprandial metabolomic response of consumers. This study aims to measure whole beef metabolomics and postprandial metabolomic response of consumers between GRA and CON beef to elucidate potential health implications.
This study followed a randomized double-blind crossover design with healthy male and female subjects ( = 10). Plasma samples were taken at fasting (0) and postprandially for four hours after consumption of a steak from each condition. Untargeted metabolomic analysis of whole beef and human plasma samples used LC/MS. Multivariate and pathway enrichment analysis in MetaboAnalyst was used to investigate metabolite and biochemical pathways that distinguished CON and GRA.
Cattle-feeding systems impacted both postprandial and whole beef steak metabolomic profiles. Metabolites that contributed to this variation included carnitine species (Proionylcarnitine), fatty acids, amino acids (L-valine), and Calamendiol. These metabolites have been associated with oxidative stress, inflammation, and cardiovascular health. Functional pathway enrichment analysis revealed numerous amino acid degradation pathways, especially branched-chain amino acids, and fatty acid degradation that changed throughout the postprandial time course.
These findings suggest that CON and GRA cattle-feeding systems differentially impact whole beef metabolomics, as well as consumer postprandial metabolic responses and the associated health implications.
背景/目的：牛的饲养系统可能对牛肉产品消费者的健康产生影响。有机草饲（GRA）和传统（CON）牛饲养系统可能导致牛肉产品具有不同的代谢物谱，因此可能影响消费者的餐后代谢组学反应。本研究旨在测量GRA牛肉和CON牛肉之间的全牛肉代谢组学以及消费者的餐后代谢组学反应，以阐明潜在的健康影响。
本研究采用随机双盲交叉设计，纳入健康男性和女性受试者（n = 10）。在空腹（0小时）以及食用每种情况下的牛排后4小时采集血浆样本。使用液相色谱/质谱联用技术对全牛肉和人体血浆样本进行非靶向代谢组学分析。在MetaboAnalyst中进行多变量和通路富集分析，以研究区分CON和GRA的代谢物和生化通路。
牛饲养系统影响了餐后和全牛排的代谢组学特征。导致这种差异的代谢物包括肉碱类（丙酰肉碱）、脂肪酸、氨基酸（L-缬氨酸）和卡黎醛。这些代谢物与氧化应激、炎症和心血管健康有关。功能通路富集分析显示，在餐后时间进程中，有许多氨基酸降解通路发生了变化，尤其是支链氨基酸，以及脂肪酸降解通路。
这些发现表明，CON和GRA牛饲养系统对全牛肉代谢组学、消费者餐后代谢反应以及相关健康影响有不同的作用。
