Bush Jason R, Iwuamadi Izuchukwu, Han Jun, Schibli David J, Goodlett David R, Deehan Edward C
MSP Starch Products Inc., Carberry, MB R0K 0H0, Canada.
Department of Food Science and Technology, University of Nebraska, Lincoln, NE 68588, USA.
Metabolites. 2024 Oct 5;14(10):536. doi: 10.3390/metabo14100536.
: Resistant starches, such as high-amylose maize starch and resistant potato starch (RPS), have prebiotic effects that are linked to improved metabolism at >15 g/day, but the effects at lower doses have not been reported. : We performed an exploratory post hoc analysis of free fatty acids (FFAs), bile acids (BAs), and ketone bodies in serum previously collected from a randomized, double-blind, placebo-controlled clinical trial evaluating the effects of one- and four-week consumption of 3.5 g/day RPS versus a placebo using two-way ANOVA adjusted by pFDR. Associations between week 4 changes in FFAs, BAs, and ketone bodies were assessed by Pearson's correlations. : RPS consumption reduced total FFAs relative to the placebo, including multiple unsaturated FFAs and octanedioic acid, with reductions in taurine- and glycine-conjugated secondary BAs also detected ( < 0.05). No changes in ketone bodies were observed ( > 0.05). Changes in 7-ketodeoxycholic acid ( = -0.595) and glycolithocholic acid ( = -0.471) were inversely correlated with treatment-induced reductions in FFAs for RPS but not the placebo, suggesting the effects were from the prebiotic. Shifts in β-hydroxybutyrate were further correlated with FFA changes in both treatments ( < 0.05). : These findings demonstrate that low doses of RPS positively influence fatty acid metabolism in humans, reducing circulating levels of FFA and conjugated BAs.
抗性淀粉，如高直链玉米淀粉和抗性马铃薯淀粉（RPS），具有益生元效应，当摄入量>15克/天时，这些效应与新陈代谢改善有关，但较低剂量的效应尚未见报道。我们对先前从一项随机、双盲、安慰剂对照临床试验中收集的血清中的游离脂肪酸（FFA）、胆汁酸（BA）和酮体进行了探索性事后分析，该试验使用经pFDR调整的双向方差分析评估了每天摄入3.5克RPS与安慰剂一周和四周的效果。通过Pearson相关性评估FFA、BA和酮体在第4周的变化之间的关联。与安慰剂相比，食用RPS可降低总FFA，包括多种不饱和FFA和辛二酸，同时还检测到牛磺酸和甘氨酸共轭次级BA的减少（P<0.05）。未观察到酮体的变化（P>0.05）。7-酮脱氧胆酸（r=-0.595）和甘氨石胆酸（r=-0.471）的变化与RPS而非安慰剂治疗引起的FFA降低呈负相关，表明这些效应来自益生元。两种治疗中β-羟基丁酸的变化也与FFA变化进一步相关（P<0.05）。这些发现表明，低剂量的RPS对人体脂肪酸代谢有积极影响，可降低循环中的FFA和共轭BA水平。
