Abdelnaby Elshymaa A, Alhaider Abdulrhman K, Ghoneim Ibrahim M, Emam Ibrahim A
Department of Clinical Sciences, College of Veterinary Medicine, King Faisal University, P.O. Box 400, Al-Ahsa 31982, Saudi Arabia.
Theriogenology Department, Faculty of Veterinary Medicine, Cairo University, Giza 12211, Egypt.
Vet Sci. 2024 Oct 5;11(10):479. doi: 10.3390/vetsci11100479.
This current study examined the ovarian (OA) and middle uterine arteries (MUA) blood flow under heat stress conditions regarding hormonal status (progesterone (P4), estradiol (E2), and nitric oxide metabolites (NOMs) assays). Eighteen pluriparous cows were examined, with twelve only being subjected to the natural mating as the other six animals were not bred. Pregnancy diagnosis was confirmed at day 30 by embryonic heartbeat and CL graviditatis ( = 6; pregnant), but some animals ( = 6) showed early embryonic death (EED), with the presence of control cows ( = 6). In the pregnant group, luteal diameter (cm) increased after mating, while in the pregnancy loss group it increased ( < 0.05) until reaching day 38 (1.41 ± 0.03), then decreased again. Luteal vascularity was declined in cows with EED after day 36 ( < 0.05) and reached its lowest level at day 50. P4 levels elevated in cows with EED until day 36 (13.64 ± 0.11) then declined. Both ipsilateral OA and MUA Doppler indices were declined in both groups except in cows who suffered from EED; both were elevated from day 38 until day 50 after mating. Ipsilateral peak systolic point (PSV cm/sec) of OA and MUA was elevated in both groups ( < 0.05), but in cows with EED this parameter was declined. E2 and NOMs levels were declined in cows with EED from day 40 and day 38 after mating ( < 0.05), respectively. This study provided novel data on the relations among the luteal diameter, E2, P4, and NOM levels, and luteal hemodynamics that predicts the amount of blood supply, which acts as a sensitive parameter to detect the alterations in luteal function during the first 50 days after mating.
本研究在热应激条件下，就激素状态（孕酮（P4）、雌二醇（E2）和一氧化氮代谢产物（NOMs）检测）对卵巢动脉（OA）和子宫中动脉（MUA）的血流情况进行了检查。对18头经产母牛进行了检查，其中12头仅进行自然交配，另外6头未进行配种。在第30天通过胚胎心跳和妊娠黄体（CL graviditatis）确认妊娠诊断（=6；妊娠），但一些动物（=6）出现早期胚胎死亡（EED），同时设有对照母牛（=6）。在妊娠组中，配种后黄体直径（cm）增加，而在妊娠丢失组中，黄体直径增加（<0.05），直至第38天达到（1.41±0.03），然后再次下降。在第36天后，发生EED的母牛黄体血管化程度下降（<0.05），并在第50天达到最低水平。发生EED的母牛P4水平在第36天之前升高（13.64±0.11），然后下降。除了发生EED的母牛外，两组的同侧OA和MUA多普勒指数均下降；配种后从第38天到第50天，这两个指数均升高。两组中OA和MUA的同侧收缩期峰值点（PSV cm/秒）均升高（<0.05），但发生EED的母牛该参数下降。发生EED的母牛中，E2和NOMs水平分别在配种后第40天和第38天下降（<0.05）。本研究提供了关于黄体直径、E2、P4和NOM水平以及黄体血流动力学之间关系的新数据，黄体血流动力学可预测血液供应量，是检测配种后前50天黄体功能变化的敏感参数。
