Saratsi Aikaterini, Samartzi Foteini, Tsiokos Dimitrios, Theodosiadou Ekaterini K, Panagiotidis Ioannis, Ligda Christina, Rekkas Constantinos A
Veterinary Research Institute, Hellenic Agricultural Organization-DIMITRA, ELGO Campus, Thermi, 57001 Thessaloniki, Greece.
Research Institute of Animal Science, Hellenic Agricultural Organization-DIMITRA, Paralimni, 58100 Giannitsa, Greece.
Vet Sci. 2024 Oct 11;11(10):494. doi: 10.3390/vetsci11100494.
The effect of four extenders on buck semen quality parameters was examined during a 48 h liquid storage. Semen was collected from six Skopelos bucks and diluted in the following extenders, containing: soy lecithin (SL, OviXcell), plant phospholipids (PP, AndroMed), egg yolk lecithin (EY, Steridyl), or no phospholipids (basic extender). Samples were stored at 5 °C for 48 h and assessed at 0, 24 and 48 h for viability (eosin-nigrosin), acrosome integrity (SpermBlue), membrane functional integrity (HOST), mitochondrial function (Rhodamine 123/SYBR-14/PI) and motility parameters (CASA). No significant reduction in total or progressive spermatozoa motility and mitochondrial function was observed at 24 h, whereas they all dropped significantly at 48 h, in all extenders. Spermatozoa viability, cell membrane functionality and acrosome integrity dropped progressively (0 h > 24 h > 48 h) in all groups. No significant difference among extenders was observed concerning spermatozoa mitochondrial function. Overall, spermatozoa viability, cell membrane functionality and acrosome integrity were higher in the three commercial extenders, compared to the basic extender. SL and EY extenders (OviXcell and Steridyl, respectively) preserved viability more effectively than the PP extender (AndroMed). Total motility was higher in the PP extender, compared to the SL extender. Spermatozoa acrosome integrity tended to be higher in the EY extender compared to all the other extenders. Further investigation of the protective potential of different types of cryoprotectants on liquid buck semen storage is important.
在48小时的液体保存期间，研究了四种稀释剂对公羊精液质量参数的影响。从六只斯科派洛斯公羊采集精液，并稀释于以下含有大豆卵磷脂（SL，OviXcell）、植物磷脂（PP，AndroMed）、蛋黄卵磷脂（EY，Steridyl）或不含磷脂（基础稀释剂）的稀释剂中。样本在5℃下保存48小时，并在0、24和48小时评估其活力（伊红-黑色素）、顶体完整性（SpermBlue）、膜功能完整性（HOST）、线粒体功能（罗丹明123/SYBR-14/PI）和运动参数（CASA）。在24小时时，所有稀释剂中总精子活力或渐进性精子活力以及线粒体功能均未观察到显著降低，而在48小时时它们均显著下降。所有组中精子活力、细胞膜功能和顶体完整性均逐渐下降（0小时>24小时>48小时）。在精子线粒体功能方面，各稀释剂之间未观察到显著差异。总体而言，与基础稀释剂相比，三种商业稀释剂中的精子活力、细胞膜功能和顶体完整性更高。SL和EY稀释剂（分别为OviXcell和Steridyl）比PP稀释剂（AndroMed）更有效地保持了活力。与SL稀释剂相比，PP稀释剂中的总活力更高。与所有其他稀释剂相比，EY稀释剂中的精子顶体完整性往往更高。进一步研究不同类型冷冻保护剂对公羊精液液体保存的保护潜力很重要。
