College of Animal Science and Technology, Southwest University, Rongchang, Chongqing 402460, China.
College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi 712100, P.R. China.
J Agric Food Chem. 2024 Nov 6;72(44):24260-24271. doi: 10.1021/acs.jafc.4c05383. Epub 2024 Oct 25.
Intramuscular fat (IMF) content is an economic trait in beef cattle that improves the meat quality. Studies have highlighted the correlation between long noncoding RNAs (lncRNAs) and IMF development. In this study, lncBNIP3 knockdown promoted bovine intramuscular preadipocyte differentiation. RNA-seq analysis of intramuscular preadipocytes with lncBNIP3 knockdown identified 230 differentially expressed genes. The PI3K-Akt and PPAR signaling pathways were enriched. lncBNIP3 interference promoted mRNA and protein expression of key genes in PI3K-Akt signaling pathway. LncBNIP3 interference reversed the effects of an AKT-inhibitor MK-2206 on Akt protein expression and lipid droplet accumulation, promoted mRNA and protein expression of essential genes in the PPAR signaling pathway, and ameliorated the inhibitory effects of a PPARg antagonist GW9662 on lipid accumulation. Therefore, lncBNIP3 inhibition of bovine intramuscular preadipocyte differentiation is likely mediated via the PI3K-Akt and PPAR signaling pathways. This study identified a valuable lncRNA with functional roles in IMF accumulation and revealed new strategies to improve beef quality.
肌内脂肪（IMF）含量是肉牛的一个经济性状，可改善肉质。研究强调了长链非编码 RNA（lncRNA）与 IMF 发育之间的相关性。本研究中，lncBNIP3 敲低促进了牛肌内前体脂肪细胞的分化。lncBNIP3 敲低的肌内前体脂肪细胞的 RNA-seq 分析鉴定出 230 个差异表达基因。PI3K-Akt 和 PPAR 信号通路富集。lncBNIP3 干扰促进了 PI3K-Akt 信号通路中关键基因的 mRNA 和蛋白表达。lncBNIP3 干扰逆转了 AKT 抑制剂 MK-2206 对 Akt 蛋白表达和脂滴积累的影响，促进了 PPAR 信号通路中必需基因的 mRNA 和蛋白表达，并改善了 PPARg 拮抗剂 GW9662 对脂滴积累的抑制作用。因此，lncBNIP3 抑制牛肌内前体脂肪细胞分化可能是通过 PI3K-Akt 和 PPAR 信号通路介导的。本研究鉴定了一个在 IMF 积累中具有功能作用的有价值的 lncRNA，并揭示了改善牛肉质量的新策略。
