Department of Biosciences and Bioengineering, Indian Institute of Technology Guwahati, Assam, 781039, India.
Virology. 2024 Dec;600:110272. doi: 10.1016/j.virol.2024.110272. Epub 2024 Oct 22.
Current study aim to explore Karanjin (Kar) isolated from the seeds of Pongamia pinnata as a potential antiviral polyphenol against Newcastle disease virus (NDV) through comprehensive investigations. For the in vitro study, plaque assays, gene and protein expression were used to analyse the inhibitory impact of Kar on NDV, where it reduced NDV replication as shown by a 13-fold suppression of the hemagglutinin-neuraminidase (HN) gene and decrease about 60% in virus activity. In ovo study showed that Kar mitigates NDV effects in chicken embryos. In silico studies involving molecular docking and molecular dynamics simulations showed strong binding between Kar and HN protein of NDV. Kar also influenced glucose metabolism, enhancing antiviral responses, as showed by the upregulation of GLUT1 and HEX genes through RT-qPCR and HPLC analyses. The study contributes valuable insights for future investigations into therapeutic applications of Kar against viral infections.
本研究旨在探讨从麻疯树种子中分离得到的卡兰金（Kar）作为一种潜在的抗新城疫病毒（NDV）的多酚类抗病毒药物。通过综合研究来进行体外实验，利用噬斑实验、基因和蛋白表达分析来研究 Kar 对 NDV 的抑制作用，结果表明它可以减少 NDV 的复制，血凝素神经氨酸酶（HN）基因的抑制倍数为 13，病毒活性降低约 60%。鸡胚实验表明 Kar 减轻了 NDV 对鸡胚的影响。涉及分子对接和分子动力学模拟的计算研究表明，Kar 与 NDV 的 HN 蛋白之间具有很强的结合能力。Kar 还影响葡萄糖代谢，增强抗病毒反应，通过 RT-qPCR 和 HPLC 分析显示 GLUT1 和 HEX 基因的上调。该研究为未来研究 Kar 治疗病毒感染的应用提供了有价值的见解。
