单细胞测序解析帕金森病的转录组图谱。

Single-cell sequencing insights into the transcriptional landscape of Parkinson's disease.

机构信息

Department of Neurology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou University, Zhengzhou, Henan 450052, China; The Academy of Medical Sciences of Zhengzhou University, Zhengzhou University, Zhengzhou, Henan 450052, China; Henan Key Laboratory of Cerebrovascular Diseases, The First Affiliated Hospital of Zhengzhou University, Zhengzhou University, Zhengzhou, Henan 450052, China.

出版信息

Ageing Res Rev. 2024 Dec;102:102553. doi: 10.1016/j.arr.2024.102553. Epub 2024 Oct 24.

DOI:10.1016/j.arr.2024.102553

PMID:39454761

Abstract

Parkinson's disease (PD) is the second most prevalent neurodegenerative disorder, with an unknown etiology and no specific treatment. Emerging single-cell and single-nucleus RNA sequencing (sc/snRNA-seq) technologies have become instrumental in unravelling cellular heterogeneity and characterizing molecular signatures at single-cell resolution. Single-cell T cell receptor sequencing (scTCR-seq) and single-cell B cell receptor sequencing (scBCR-seq) technologies provide unprecedented opportunities to explore the immune repertoire diversity. These state-of-the-art technologies have been increasingly applied in PD research in the last five years, offering novel insights into the cellular susceptibilities and complex molecular mechanisms underlying PD pathogenesis. Herein we review recent advances in the applications of sc/snRNA-seq, scTCR-seq and scBCR-seq technologies in various PD models. Moreover, we focus on degenerative neurons, activated neuroglial cells, as well as pro-inflammatory immune cells, exploring their unique transcriptional landscapes in PD, as revealed by single-cell sequencing technologies. Finally, we highlight important challenges and the future directions of single-cell experiments in PD research.

摘要

帕金森病（PD）是第二大常见的神经退行性疾病，其病因不明，也没有特定的治疗方法。新兴的单细胞和单细胞核 RNA 测序（sc/snRNA-seq）技术在揭示细胞异质性和单细胞分辨率下的分子特征方面发挥了重要作用。单细胞 T 细胞受体测序（scTCR-seq）和单细胞 B 细胞受体测序（scBCR-seq）技术为探索免疫受体多样性提供了前所未有的机会。这些最先进的技术在过去五年中越来越多地应用于 PD 研究，为 PD 发病机制中的细胞易感性和复杂分子机制提供了新的见解。本文综述了 sc/snRNA-seq、scTCR-seq 和 scBCR-seq 技术在各种 PD 模型中的应用的最新进展。此外，我们重点关注退行性神经元、激活的神经胶质细胞以及促炎免疫细胞，通过单细胞测序技术探索它们在 PD 中的独特转录景观。最后，我们强调了 PD 研究中单细胞实验的重要挑战和未来方向。