Key Laboratory of Plant Resource Conservation and Germplasm Innovation in Mountainous Region (Ministry of Education), Collaborative Innovation Center for Mountain Ecology and Agro-Bioengineering (CICMEAB), College of Life Sciences, Guizhou University, Huaxi District, Guiyang, 550025, Guizhou, China.
Institute of Fungal Resources, College of Life Sciences, Guizhou University, Institute of Agricultural Bioengineering, Guizhou University, Guiyang, 550025, Guizhou, China.
Sci Rep. 2024 Oct 25;14(1):25369. doi: 10.1038/s41598-024-74448-8.
Ectomycorrhizal fungi (EMF) are key symbiotic microbial components for the growth and health of trees in urban greenspace habitats (UGSHs). However, the current understanding of EMF diversity in UGSHs remains poor. Therefore, in this study, using morphological classification and molecular identification, we aimed to investigate EMF diversity in three EMF host plants: Cedrus deodara in the roadside green belt, and C. deodara, Pinus massoniana, and Salix babylonica in the park roadside green belt, in Guiyang, China. A total of 62 EMF Operational Taxonomic Units (OTUs) were identified, including 13 EMF OTUs in the C. deodara roadside green belt, and 23, 31, and 9 EMF OTUs in the park green belts. C. deodara, P. massoniana, and S. babylonica were respectively identified in park green belts. Ascomycota and Basidiomycota were the dominant phylum in the EMF communities in roadside and park green habitat, respectively. The Shannon and Simpson indexes of the C. deodara EMF community in the park green belt were higher than those in the roadside green belt. EMF diversity of the tree species in the park green belt was P. massoniana > C. deodara > S. babylonica. Differences in EMF community diversity was observed among the different greening tree species in the UGSHs. UGSHs with different disturbance gradients had a significant impact on the EMF diversity of the same greening tree species. These results can be used as a scientific reference for optimizing the design and scientific management of UGSHs.
外生菌根真菌（ectomycorrhizal fungi，EMF）是城市绿地生境（UGSH）中树木生长和健康的关键共生微生物组成部分。然而，目前对 UGSH 中 EMF 多样性的了解仍然很少。因此，在这项研究中，我们使用形态分类和分子鉴定方法，旨在调查中国贵阳三种 EMF 宿主植物（路边绿化带中的雪松、公园绿化带中的雪松、马尾松和垂柳）中的 EMF 多样性。共鉴定出 62 个 EMF 操作分类单元（OTUs），包括路边绿化带中的雪松 13 个 EMF OTUs，公园绿化带中的马尾松 23 个、垂柳 31 个和 9 个 EMF OTUs。公园绿化带中鉴定出雪松、马尾松和垂柳。子囊菌门和担子菌门分别是路边和公园绿地 EMF 群落中的优势门。公园绿地中雪松 EMF 群落的 Shannon 和 Simpson 指数高于路边绿化带。公园绿地中不同绿化树种的 EMF 多样性为马尾松 > 雪松 > 垂柳。不同 UGSH 中绿化树种的 EMF 群落多样性存在差异。具有不同干扰梯度的 UGSH 对同一绿化树种的 EMF 多样性有显著影响。这些结果可以为优化 UGSH 的设计和科学管理提供科学参考。
