Guangxi International Zhuang Medicine Hospital Affiliated to Guangxi University of Chinese Medicine, Nanning, 530001, China.
Operating Room, North China University of Science and Technology Affiliated Hospital, Tangshan, 063000, China.
BMC Endocr Disord. 2024 Oct 25;24(1):225. doi: 10.1186/s12902-024-01755-6.
To explore the association of long non-coding RNA (lncRNA) SNHG1/ miR-195 axis with type 2 diabetes mellitus (T2DM) and islet function.
The expression of SNHG1 and miR-195 was measured in T2DM patients and in healthy subjects. Correlation between indciators was evaluated using Pearson correlation analysis. INS-1 cells were used to perform the cell function assays. Insulin secretion by INS-1 was detected using ELISA. Cell counting kit-8 (CCK-8) and flow cytometry was used to detect cell proliferation and apoptosis. Luciferase report assay was to used to verify the target of SNHG1.
The expression of SNHG1 was increased and miR-195 level was decreased in the serum of T2DM patients. Both SNHG1 and miR-195 could be biomarkers for T2DM diagnosis. The fasting plasma glucose (FPG) and HbA1c were positively related to SNHG1 and negatively related to miR-195. SNHG1 inhibited insulin secretion, and cell proliferation and promoted apoptosis of INS-1 cells via binding to miR-195.
Detection of SNHG1 and miR-195 might predict T2DM. SNHG1 could suppress proliferation and insulin secretion, but promote apoptosis of INS-1 cells via sponging miR-195.
探讨长链非编码 RNA（lncRNA）SNHG1/miR-195 轴与 2 型糖尿病（T2DM）及胰岛功能的关系。
检测 T2DM 患者和健康人群中 SNHG1 和 miR-195 的表达。采用 Pearson 相关分析评估各指标间的相关性。使用 INS-1 细胞进行细胞功能测定。采用 ELISA 法检测 INS-1 的胰岛素分泌。用细胞计数试剂盒-8（CCK-8）和流式细胞术检测细胞增殖和凋亡。采用荧光素酶报告实验验证 SNHG1 的靶基因。
T2DM 患者血清中 SNHG1 表达增加，miR-195 水平降低。SNHG1 和 miR-195 均可作为 T2DM 诊断的生物标志物。空腹血糖（FPG）和糖化血红蛋白（HbA1c）与 SNHG1 呈正相关，与 miR-195 呈负相关。SNHG1 通过与 miR-195 结合抑制 INS-1 细胞的胰岛素分泌、增殖，并促进其凋亡。
检测 SNHG1 和 miR-195 可能有助于预测 T2DM。SNHG1 可能通过海绵吸附 miR-195 抑制 INS-1 细胞的增殖和胰岛素分泌，但促进其凋亡。
