Fields M, Holbrook J, Scholfield D, Rose A, Smith J C, Reiser S
Proc Soc Exp Biol Med. 1986 Jan;181(1):120-4. doi: 10.3181/00379727-181-42232.
Copper deficiency was induced in weanling rats fed diets whose sole source of carbohydrates was starch or fructose for 7 weeks. Conventional parameters of copper status, plasma copper concentrations, ceruloplasmin activity, and erythrocyte superoxide dismutase (SOD) activity were longitudinally monitored weekly to follow the development of the deficiency and to correlate these indices with the degree of severity of the deficiency. Although 30% of the rats fed a copper-deficient fructose diet died and no deaths occurred in rats fed the copper-deficient starch diet, plasma copper, ceruloplasmin, and SOD activities were reduced to a similar extent in all rats fed copper-deficient diets regardless of the type of dietary carbohydrate. Thus, none of the indices used accurately reflected the greater degree of deficiency or mortality in rats fed the fructose diet deficient in copper. The results of the present study underscore the need for more sensitive tests or alternative parameters to assess copper status in living animals.
给断乳大鼠喂食以淀粉或果糖作为唯一碳水化合物来源的饲料7周，从而诱发铜缺乏。每周纵向监测铜状态的常规参数、血浆铜浓度、铜蓝蛋白活性和红细胞超氧化物歧化酶（SOD）活性，以跟踪缺乏症的发展，并将这些指标与缺乏症的严重程度相关联。尽管30%喂食缺铜果糖饲料的大鼠死亡，而喂食缺铜淀粉饲料的大鼠未出现死亡，但无论膳食碳水化合物的类型如何，所有喂食缺铜饲料的大鼠的血浆铜、铜蓝蛋白和SOD活性均降低到相似程度。因此，所使用的指标均未准确反映喂食缺铜果糖饲料的大鼠中更严重的缺乏程度或死亡率。本研究结果强调需要更敏感的测试或替代参数来评估活体动物的铜状态。
