Georgetown University Medical School, 20007, Washington, DC, USA.
Biol Trace Elem Res. 1986 Oct;10(4):335-46. doi: 10.1007/BF02802401.
The present investigation was designed to study the uptake of(67)Cu when administered directly, into the portal vein, along with either functose or glucose, by the liver and extrahepatic tissues. Following weaning, male Sprague-Dawley rats were fed for 3 wk either commercial laboratory ration (chow) or semipurified diets deficient in Cu (0.6 ppm) or supplemented with Cu (6.0 ppm) and containing 62% carbohydrate as either fructuse or cornstarch. After an overnight fast, a single dose of rat plasma (0.1 mL) containing fructose or glucose extrinsically labeled with(67)Cu was injected directly into their portal vein. Although not always statistically significant, rats fed chow retained more radioactivity in the liver and several extrahepatic tissues when(67)Cu was administered with fructose than with glucose. Regardless of Cu status, rats fed diets containing fructose retained more radioactivity in extrahepatic tissues than rats fed starch. There was an increased uptake of(67)Cu by the liver, blood, muscle, and fat pad when fructose as compared to glucose was injected in combination with the isotope. These data strongly suggest that Cu requirements or utilization are greater when fructose is the main dietary carbohydrate. The results may also in part explain the reason for the increased severity of Cu deficiency in rats fed fructose.
本研究旨在研究当（67）Cu 直接通过门静脉给药时，与果糖或葡萄糖一起被肝脏和肝外组织摄取的情况。断奶后，雄性 Sprague-Dawley 大鼠连续 3 周分别喂食商业实验室饲料（标准饲料）或缺乏铜（0.6ppm）的半纯化饲料，或补充铜（6.0ppm），并含有 62%的碳水化合物，其形式为果糖或玉米淀粉。在禁食过夜后，将含有果糖或葡萄糖的大鼠血浆（0.1ml）的单一剂量直接注入门静脉，该血浆通过（67）Cu 进行了外源性标记。尽管并不总是具有统计学意义，但与葡萄糖相比，当（67）Cu 与果糖一起给药时，喂食标准饲料的大鼠在肝脏和几个肝外组织中保留更多的放射性。无论铜状态如何，喂食含果糖饮食的大鼠在肝外组织中保留的放射性高于喂食淀粉的大鼠。与注射葡萄糖相比，当将果糖与同位素一起注射时，肝脏、血液、肌肉和脂肪垫对（67）Cu 的摄取量增加。这些数据强烈表明，当果糖是主要膳食碳水化合物时，铜的需求或利用率更高。这些结果也可能部分解释了喂食果糖的大鼠铜缺乏症加重的原因。
