School of Electrical Engineering, Shandong University, Ji'nan 250061, China.
Biomolecules. 2024 Sep 25;14(10):1207. doi: 10.3390/biom14101207.
Non-thermal plasma (NTP) synergistic anticancer strategies are a current hotspot of interest at the intersection of plasma biomedicine. Melittin (MEL) has been shown to inhibit cancer in many malignant tumors; however, its clinical application is controversial. Therefore, the transmembrane process and mechanism of MEL activity in different cell systems were studied and the combination of MEL and NTP was proposed in this paper. The results showed that the electrostatic attraction between MEL and the lipid bilayer contributes to the stable orientation of MEL on the membrane surface. In addition, sialic acid overexpression affects the degree to which MEL binds the membrane system and the stability of the membrane structure. The use of NTP to reduce the dosage of MEL and its related nonspecific cytolysis activity has certain clinical application value. The results of this study provide theoretical support for improving the clinical applicability of MEL and contribute to the further development of plasma biomedicine.
非热等离子体（NTP）协同抗癌策略是等离子体医学交叉领域的当前热点。蜂毒素（MEL）已被证明能抑制许多恶性肿瘤中的癌症；然而，其临床应用仍存在争议。因此，本研究旨在探讨 MEL 在不同细胞系统中的跨膜过程和作用机制，并提出 MEL 与 NTP 的联合应用。结果表明，MEL 与脂质双层之间的静电吸引有助于 MEL 在膜表面的稳定取向。此外，唾液酸的过度表达影响 MEL 与膜系统的结合程度以及膜结构的稳定性。利用 NTP 降低 MEL 的剂量及其相关的非特异性细胞溶解活性具有一定的临床应用价值。本研究结果为提高 MEL 的临床适用性提供了理论支持，有助于推动等离子体生物医学的进一步发展。
