Ferri Gianluigi, Pennisi Luca, Malatesta Filiberto, Vergara Alberto
Department of Veterinary Medicine, Post-Graduate Specialization School in Food Inspection "G. Tiecco", University of Teramo, Strada Provinciale 18, 64100 Teramo, Italy.
Food and Health Veterinarian Consulting, 64100 Teramo, Italy.
Foods. 2024 Oct 10;13(20):3218. doi: 10.3390/foods13203218.
HEV mainly enters animal and human hosts through the orofecal route, which presents a critical health concern alongside the associated environmental variable. Among products of animal origin, milk (both ovine and bovine) can harbor HEV RNA, which can potentially be transmitted to consumers. In this study, a total of 220 raw ovine milk samples were collected from Apennine breed subjects farmed (transhumance method) in three different Italian provinces, L'Aquila, Pescara, and Teramo, located in the Abruzzo region (Central Italy). All the specimens were screened using one-step real-time RT-qPCR and nested RT-PCR assays. Among them, 5/220 or 2.27% harbored HEV RNA fragments belonging to the ORF1 and ORF2 codifying regions of the genotype 3c. The average viral amount discovered was 10 GE/mL. These subjects represented 2/57 or 3.51% of the Pescara herd, and 3/105 or 2.86% of the Teramo herd. Although HEV RNA was discovered in sheep fecal samples, the original data obtained in the present study represent the first HEV RNA detection in ovine raw milk from Italy.
戊型肝炎病毒主要通过粪口途径进入动物和人类宿主，这与相关的环境变量一起构成了一个关键的健康问题。在动物源性产品中，牛奶（羊乳和牛乳）可能携带戊型肝炎病毒RNA，有可能传染给消费者。在本研究中，从位于意大利中部阿布鲁佐地区的拉奎拉、佩斯卡拉和泰拉莫三个不同省份采用转场放牧方式养殖的亚平宁品种羊身上共采集了220份生羊乳样本。所有样本均采用一步法实时逆转录定量聚合酶链反应和巢式逆转录聚合酶链反应检测。其中，5/220（2.27%）样本携带属于3c基因型开放阅读框1和开放阅读框2编码区的戊型肝炎病毒RNA片段。发现的平均病毒量为10基因组当量/毫升。这些样本分别占佩斯卡拉羊群的2/57（3.51%）和泰拉莫羊群的3/105（2.86%）。虽然在绵羊粪便样本中发现了戊型肝炎病毒RNA，但本研究获得的原始数据代表了意大利首次在生羊乳中检测到戊型肝炎病毒RNA。
