Sun Yanan, Wen Tianjian, Zhang Ping, Wang Minglian, Xu Yuancong
College of Chemistry and Life Science, Beijing University of Technology, Beijing 100124, China.
Foods. 2024 Oct 10;13(20):3222. doi: 10.3390/foods13203222.
Food safety is a major public health issue of global concern. In recent years, the CRISPR/Cas system has shown promise in the field of molecular detection. The system has been coupled with various nucleic acid amplification methods and combined with different signal output systems to develop a new generation of CRISPR/Cas-based nucleic acid biosensor technology. This review describes the design concept of the CRISPR/Cas-based nucleic acid biosensor and its application in food analysis. A detailed overview of different CRISPR/Cas systems, signal amplification methods, and signal output strategies is provided. CRISPR/Cas-based nucleic acid biosensors have the advantages of high sensitivity, strong specificity, and timeliness, achieving fast analysis of a variety of targets, including bacteria, toxins, metal ions, pesticides, veterinary drugs, and adulteration, promoting the development of rapid food safety detection technology. At the end, we also provide our outlook for the future development of CRISPR/Cas-based nucleic acid biosensors.
食品安全是一个全球关注的重大公共卫生问题。近年来，CRISPR/Cas系统在分子检测领域展现出了应用前景。该系统已与多种核酸扩增方法相结合，并与不同的信号输出系统联合，以开发新一代基于CRISPR/Cas的核酸生物传感器技术。本综述描述了基于CRISPR/Cas的核酸生物传感器的设计理念及其在食品分析中的应用。文中提供了不同CRISPR/Cas系统、信号放大方法和信号输出策略的详细概述。基于CRISPR/Cas的核酸生物传感器具有高灵敏度、强特异性和及时性的优点，能够对包括细菌、毒素、金属离子、农药、兽药和掺假物在内的多种目标进行快速分析，推动了食品安全快速检测技术的发展。最后，我们还对基于CRISPR/Cas的核酸生物传感器的未来发展进行了展望。
