Qin Ying-Chao, Jin Cheng-Long, Hu Ting-Cai, Zhou Jia-Yi, Wang Xiao-Fan, Wang Xiu-Qi, Kong Xiang-Feng, Yan Hui-Chao
State Key Laboratory of Swine and Poultry Breeding Industry, Guangdong Laboratory for Lingnan Modern Agriculture, Guangdong Provincial Key Laboratory of Animal Nutrition Control, National Engineering Research Center for Breeding Swine Industry, College of Animal Science, South China Agricultural University, Guangzhou 510642, China.
Key Laboratory of Animal Nutrition and Feed Science in South China, State Key Laboratory of Swine and Poultry Breeding Industry, Guangdong Provincial Key Laboratory of Animal Breeding and Nutrition, Institute of Animal Science, Guangdong Academy of Agricultural Sciences, Ministry of Agriculture and Rural Affairs, Guangzhou 510640, China.
Antioxidants (Basel). 2024 Sep 30;13(10):1188. doi: 10.3390/antiox13101188.
There are different stress resistance among different breeds of pigs. Changes in intestinal stem cells (ISCs) are still unclear among various breeds of piglets after early weaning. In the current study, Taoyuan Black and Duroc piglets were slaughtered at 21 days of age (early weaning day) and 24 days of age (3 days after early weaning) for 10 piglets in each group. The results showed that the rate of ISC-driven epithelial renewal in local Taoyuan Black pigs hardly changed after weaning for 3 days. However, weaning stress significantly reduced the weight of the duodenum and jejunum in Duroc piglets. Meanwhile, the jejunal villus height, tight junction-related proteins (ZO-1, Occludin, and Claudin1), as well as the trans-epithelial electrical resistance (TEER) values, were down-regulated after weaning for 3 days in Duroc piglets. Moreover, compared with Unweaned Duroc piglets, the numbers of Olfm4 ISC cells, PCNA mitotic cells, SOX9 secretory progenitor cells, and Villin absorptive cells in the jejunum were reduced significantly 3 days after weaning. And ex vivo jejunal crypt-derived organoids exhibited growth disadvantages in weaned Duroc piglets. Notably, the Keap1/Nrf2 signaling activities and the expression of HO-1 were significantly depressed in weaned Duroc piglets compared to Unweaned Duroc piglets. Thus, we can conclude that ISCs of Duroc piglets were more sensitive to weaning stress injury than Taoyuan Black piglets, and Keap1/Nrf2 signaling is involved in this process.
不同品种的猪具有不同的抗应激能力。早期断奶后，不同品种仔猪肠道干细胞（ISC）的变化仍不清楚。在本研究中，每组10头仔猪，分别在21日龄（早期断奶日）和24日龄（早期断奶后3天）屠宰桃源黑猪和杜洛克猪。结果表明，桃源黑猪断奶3天后，ISC驱动的局部上皮更新率几乎没有变化。然而，断奶应激显著降低了杜洛克仔猪十二指肠和空肠的重量。同时，杜洛克仔猪断奶3天后，空肠绒毛高度、紧密连接相关蛋白（ZO-1、闭合蛋白和Claudin1）以及跨上皮电阻（TEER）值均下调。此外，与未断奶的杜洛克仔猪相比，断奶3天后空肠中Olfm4 ISC细胞、PCNA有丝分裂细胞、SOX9分泌祖细胞和Villin吸收细胞的数量显著减少。断奶的杜洛克仔猪空肠隐窝来源的类器官在体外培养时表现出生长劣势。值得注意的是，与未断奶的杜洛克仔猪相比，断奶的杜洛克仔猪中Keap1/Nrf2信号活性和HO-1的表达显著降低。因此，我们可以得出结论，杜洛克仔猪的ISC比桃源黑猪对断奶应激损伤更敏感，并且Keap1/Nrf2信号通路参与了这一过程。
