Swine Disease Research Center, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China.
Key Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Chengdu 611130, China.
Int J Mol Sci. 2024 Oct 10;25(20):10878. doi: 10.3390/ijms252010878.
Porcine epidemic diarrhea (PED) is an acute, highly contagious, and infectious disease caused by porcine epidemic diarrhea virus (PEDV). PEDV can affect pigs of all ages, with 50~100% mortality in neonatal piglets and substantial economic losses in the swine industry. In the present study, 347 fecal and intestinal samples were collected from seven regions in China during 2020-2022. A comprehensive molecular investigation of the spike (S) gene of PEDV strains was carried out, which included phylogenetic analysis of the obtained PEDV sequences. Epidemiological surveillance data indicate that the GIIc subgroup strains are widely distributed among pigs. A PEDV strain was successfully isolated from positive small intestine samples and identified through RT-PCR detection using specific N gene primers of PEDV, indirect immunofluorescence assay (IFA), TEM analysis, genome sequencing, and full-length S gene analysis, named PEDV/SC/2022. RDP and SimPlot analysis showed that the isolate originated from the recombination of PEDV/AH2012 and PEDV/AJ1102. In conclusion, our findings contribute to the current understanding of PEDV epidemiology and provide valuable information for the control of PED outbreaks in China.
猪流行性腹泻（PED）是由猪流行性腹泻病毒（PEDV）引起的一种急性、高度传染性和感染性疾病。PEDV 可感染所有年龄段的猪，仔猪的死亡率为 50%~100%，给养猪业造成了巨大的经济损失。本研究于 2020-2022 年期间，从中国七个地区采集了 347 份粪便和肠道样本。对采集到的 PEDV 序列进行了系统分子流行病学调查，包括对 PEDV 刺突（S）基因进行了系统发育分析。通过对 PEDV 分离株的 S 基因全长序列分析，结果显示，PEDV 在中国流行的主要基因型为 GIIc 亚群，且在不同地区、不同时间的猪群中均有流行。从阳性小肠组织中分离到一株 PEDV 病毒，经 RT-PCR 检测、间接免疫荧光试验（IFA）、电镜观察、基因组测序和全长 S 基因分析鉴定为 PEDV，命名为 PEDV/SC/2022。RDP 和 SimPlot 分析表明，该分离株来源于 PEDV/AH2012 和 PEDV/AJ1102 的重组。本研究结果有助于了解 PEDV 的流行情况，为中国 PED 的防控提供了有价值的信息。
