Key Laboratory of Northwest Facility Horticulture Engineering of Ministry of Agriculture and Rural Affairs, College of Horticulture, Northwest A & F University, Yangling 712000, China.
College of Life Sciences, Northwest A & F University, Yangling 712000, China.
Int J Mol Sci. 2024 Oct 14;25(20):11031. doi: 10.3390/ijms252011031.
The gene, a member of the (xyloglucan endotransglycosylase/hydrolase) family, plays a crucial role in plant responses to environmental stress. In this study, we heterologously expressed the melon gene in to generate overexpressing transgenic lines, thereby elucidating the regulatory role of in water stress tolerance. Using these lines of (OE1 and OE2) and wild-type (WT) as experimental materials, we applied water stress treatments (including osmotic stress and soil drought) and rewatering treatments to investigate the response mechanisms of melon in under drought stress from a physiological and biochemical perspective. Overexpression of significantly improved root growth under water stress conditions. The OE lines exhibited longer roots and a higher number of lateral roots compared to WT plants. The enhanced root system contributed to better water uptake and retention. Under osmotic and drought stress, the OE lines showed improved survival rates and less wilting compared to WT plants. Biochemical analyses revealed that overexpression led to lower levels of malondialdehyde (MDA) and reduced electrolyte leakage, indicating decreased oxidative damage. The activities of antioxidant enzymes, including superoxide dismutase (SOD), catalase (CAT), and peroxidase (POD), were significantly higher in OE lines, suggesting enhanced oxidative stress tolerance. The gene positively regulates water stress tolerance in by enhancing root growth, improving water uptake, and reducing oxidative damage. Overexpression of increases the activities of antioxidant enzymes, thereby mitigating oxidative stress and maintaining cellular integrity under water deficit conditions. These findings suggest that is a potential candidate for genetic improvement of drought resistance in crops.
该基因是（木葡聚糖内转糖基酶/水解酶）家族的成员，在植物对环境胁迫的响应中起着关键作用。在这项研究中，我们在拟南芥中异源表达了甜瓜基因，以产生过表达的转基因系，从而阐明了在水胁迫耐受性中甜瓜的调控作用。使用这些过表达系（OE1 和 OE2）和野生型（WT）作为实验材料，我们施加了水分胁迫处理（包括渗透胁迫和土壤干旱）和复水处理，从生理和生化角度研究了甜瓜在干旱胁迫下的响应机制。过表达 显著改善了水分胁迫条件下的根系生长。OE 系的根长较长，侧根数较多，与 WT 植物相比。增强的根系有助于更好地吸收和保持水分。在渗透胁迫和干旱胁迫下，OE 系的存活率高于 WT 植物，萎蔫程度较低。生化分析表明，过表达导致丙二醛（MDA）水平降低和电解质泄漏减少，表明氧化损伤减轻。超氧化物歧化酶（SOD）、过氧化氢酶（CAT）和过氧化物酶（POD）等抗氧化酶的活性在 OE 系中显著升高，表明对氧化应激的耐受性增强。该基因通过增强根系生长、提高水分吸收和减少氧化损伤，正向调控甜瓜在水分胁迫下的耐受性。过表达增加了抗氧化酶的活性，从而减轻了水分亏缺条件下的氧化应激并维持了细胞完整性。这些发现表明 是作物抗旱遗传改良的潜在候选基因。
