College of Life Science, Northeast Agricultural University, Harbin 150030, China.
Key Laboratory of Soybean Molecular Breeding, Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, Harbin 150081, China.
Int J Mol Sci. 2024 Oct 19;25(20):11253. doi: 10.3390/ijms252011253.
Sulfur is a critical element for plant growth and development, serving as a component of amino acids (cysteine and methionine), iron-sulfur clusters, proteins, glutathione, coenzymes, and auxin precursors. Deficiency or low concentrations of sulfur in the soil can lead to significant growth retardation in plants. The objective of our study was to examine the effects of sulfur (S) deficiency and excess on morphological symptoms, sulfur and nitrogen (N) metabolism, as well as antioxidant activity in soybean. We found that S starvation decreased the fine root length, biomass, and activity, and the chlorophyll content was reduced, while excess sulfur promotes lateral root growth. In contrast to sulfur excess, sulfur deficiency inhibits N and S metabolism levels in both subsurface and above-ground parts, and induced the expression of some sulfur transporters (SULTRs). In this study, we created soybean hairy root lines overexpressing the SULTR gene () to observe metabolic changes following sulfur deficiency treatment. The results showed that saved the sulfur-deficient phenotype, and the antioxidant enzyme activity was much higher than that of the wildtype in the absence of sulfur. Our study revealed the important role of sulfur element in soybean growth and development and the regulation of sulfur deficiency by .
硫是植物生长和发育的关键元素，是氨基酸（半胱氨酸和蛋氨酸）、铁硫簇、蛋白质、谷胱甘肽、辅酶和生长素前体的组成部分。土壤中硫的缺乏或浓度低会导致植物生长显著减缓。我们的研究目的是研究硫（S）缺乏和过量对大豆形态症状、硫和氮（N）代谢以及抗氧化活性的影响。我们发现 S 饥饿会降低细根长度、生物量和活性，降低叶绿素含量，而过量的硫会促进侧根生长。与硫过量相反，硫缺乏抑制地下和地上部分的氮和硫代谢水平，并诱导一些硫转运蛋白（SULTRs）的表达。在这项研究中，我们创建了过量表达 SULTR 基因（）的大豆毛状根系，以观察硫缺乏处理后的代谢变化。结果表明，挽救了硫缺乏表型，在没有硫的情况下，抗氧化酶活性比野生型高得多。我们的研究揭示了硫元素在大豆生长发育中的重要作用以及在硫缺乏时对的调节作用。
