College of Life Science, Northeast Agricultural University, Harbin 150001, China.
Int J Mol Sci. 2024 Aug 21;25(16):9080. doi: 10.3390/ijms25169080.
Sulfate transporters (SULTRs) are essential for the transport and absorption of sulfate in plants and serve as critical transport proteins within the sulfur metabolism pathway, significantly influencing plant growth, development, and stress adaptation. A bioinformatics analysis of SULTR genes in soybean was performed, resulting in the identification and classification of twenty-eight putative GmSULTRs into four distinct groups. In this study, the characteristics of the 28 genes, including those involved in collinearity, gene structure, protein motifs, -elements, tissue expression patterns, and the response to abiotic stress and plant hormone treatments, were systematically analyzed. This study focused on conducting a preliminary functional analysis of the gene, wherein a high expression level of in the roots, stems, and leaves was induced by a sulfur deficiency and improved the salt tolerance. A further functional characterization revealed that overexpressing soybean hairy roots had higher SO, GSH, and methionine (Met) contents compared with the wild-type (WT) plant. These results demonstrate that the overexpression of may promote the sulfur assimilation metabolism and increase the content of sulfur-containing amino acids in plants.
硫酸盐转运蛋白（SULTRs）是植物中硫酸盐运输和吸收所必需的，它们作为硫代谢途径中的关键转运蛋白，显著影响植物的生长、发育和应激适应。本研究对大豆中的 SULTR 基因进行了生物信息学分析，鉴定并分类了 28 个假定的 GmSULTRs，分为四个不同的组。在这项研究中，对 28 个基因的特征进行了系统分析，包括基因的共线性、基因结构、蛋白质基序、顺式作用元件、组织表达模式以及对非生物胁迫和植物激素处理的响应。本研究侧重于对基因进行初步的功能分析，结果表明，在根、茎和叶中，基因的表达水平在硫缺乏时显著升高，而过表达基因可提高植物的耐盐性。进一步的功能特征表明，与野生型（WT）植物相比，过表达大豆毛状根具有更高的 SO42-、GSH 和蛋氨酸（Met）含量。这些结果表明，过表达基因可能促进了植物的硫同化代谢，增加了含硫氨基酸的含量。
