Gansu Provincial Key Laboratory of Aridland Crop Science, College of Life Science and Technology, Gansu Agricultural University, Lanzhou 730070, China.
Cotton Research Institute, Xinjiang Academy of Agricultural and Reclamation Science, Shihezi 832000, China.
Int J Mol Sci. 2024 Oct 21;25(20):11321. doi: 10.3390/ijms252011321.
The trithorax group (TrxG) complex is an important protein in the regulation of plant histone methylation. The ABSENT, SMALL, OR HOMEOTIC DISCS 1 (ASH1) gene family, as important family members of the TrxG complex, has been shown to regulate tolerance to abiotic stress and growth and development in many plants. In this study, we identified nine in upland cotton. Bioinformatics analysis revealed that contain a variety of cis-acting elements related to stress resistance and growth and development. The transcriptome expression profiles revealed that and genes expression were upregulated in flower organs and in response to external temperature stress. The results of virus-induced gene silencing (VIGS) indicated that and genes silencing reduced the ability of cotton to adapt to temperature stress and delayed the development of the flowering phenotype. We also showed that the silencing of these two target genes did not induce early flowering at high temperature (32 °C), suggesting that and might regulate cotton flowering in response to temperature. These findings provide genetic resources for future breeding of early-maturing and temperature-stress-tolerant cotton varieties.
三价组（TrxG）复合物是调节植物组蛋白甲基化的重要蛋白质。作为 TrxG 复合物的重要成员之一，缺失、矮小、同源异形盘 1（ASH1）基因家族已被证明在许多植物中调节非生物胁迫耐受性和生长发育。在本研究中，我们在陆地棉中鉴定出了 9 个。生物信息学分析表明， 包含与抗逆性和生长发育相关的多种顺式作用元件。转录组表达谱显示， 和 基因在花器官中的表达上调，并对环境温度胁迫有响应。病毒诱导的基因沉默（VIGS）的结果表明， 和 基因沉默降低了棉花适应温度胁迫的能力，并延迟了开花表型的发育。我们还表明，这两个靶基因的沉默并没有在高温（32°C）下诱导提前开花，这表明 和 可能通过响应温度调节棉花的开花。这些发现为未来培育早熟和耐温棉花品种提供了遗传资源。
