Yunnan Key Laboratory of Green Prevention and Control of Agricultural Transboundary Pests, The Ministry of Agriculture and Rural Affairs International Joint Research Center for Agriculture, Agricultural Environment and Resource Research Institute, Yunnan Academy of Agricultural Sciences, Kunming 650205, China.
Flower Research Institute, Yunnan Academy of Agricultural Sciences, Kunming 650205, China.
Genes (Basel). 2024 Oct 15;15(10):1325. doi: 10.3390/genes15101325.
: Rice blast, caused by , seriously damages the yield and quality of rice worldwide. is a durable resistance gene that combats carrying . However, the distribution of the gene in rice germplasms in Yunnan Province has been inadequately studied. : We analyzed the potential molecular evolution pattern of alleles by examining the diversity in the coding sequence (CDS) among rice varieties. : The results revealed that 95% of 405 rice landraces collected from different ecological regions in Yunnan Province carry alleles. We identified 17 nucleotide variation sites in the CDS regions of the gene across 385 rice landraces. These variations led to the identification of 28 haplotypes, encoding 12 novel variants. Among these, 5 haplotypes (62 rice landraces) carried alleles. The evolutionary cluster and network of the haplotypes suggested that the alleles were the ancestral alleles, which could potentially evolve into variants through base substitution. : This study suggests that alleles are diverse in the rice landraces in Yunnan, and the sites resistant to blast evolved from the susceptible plants of the rice landraces. These results provide the basis for breeding resistant varieties.
稻瘟病由 引起，严重损害了全球水稻的产量和质量。 是一种持久的抗性基因，可抵抗携带 的病原菌。然而，云南省水稻种质资源中 基因的分布情况尚未得到充分研究。
我们通过检查不同水稻品种中编码序列（CDS）的多样性来分析 等位基因的潜在分子进化模式。结果表明，云南省不同生态区采集的 405 份水稻地方品种中有 95%携带 等位基因。我们在 385 份水稻地方品种的 CDS 区域中鉴定出 17 个核苷酸变异位点。这些变异导致了 28 个 基因的单倍型，编码 12 个新的变异体。其中，5 个单倍型（62 份水稻地方品种）携带 等位基因。 单倍型的进化聚类和网络图表明， 等位基因在云南水稻地方品种中具有多样性，而对稻瘟病具有抗性的 位点可能是由水稻地方品种的感病植物进化而来的。这些结果为培育抗性品种提供了依据。
