Rembe Julian-Dario, Garabet Waseem, Lackmann Jan-Wilm, Alizadehrahrouei Sadaf, Augustin Matthias, Dissemond Joachim, Ibing Wiebke, Köhrer Karl, Pfeffer Klaus, Rommerskirchen Anna, Scharf Sebastian Alexander, Wienemann Tobias, Wachtmeister Thorsten, Schelzig Hubert, Stuermer Ewa Klara
Department for Vascular and Endovascular Surgery, University Hospital Duesseldorf (UKD), Heinrich Heine University Duesseldorf, 40225 Duesseldorf, Germany.
Cologne Excellence Cluster for Cellular Stress Responses in Aging-Associated Diseases (CECAD), University of Cologne, 50923 Cologne, Germany.
Biomedicines. 2024 Sep 26;12(10):2187. doi: 10.3390/biomedicines12102187.
Patient-specific diagnostic and therapeutic approaches are important in the care of people with chronic wounds. The heterogeneity of underlying disease profiles and the diversity of the wound micro-environment make generalized approaches difficult. While high-throughput molecular diagnostic methods are increasingly widespread and available, the analysis of objective biomolecular disease patterns has not found its way into everyday wound management. The aim of this study is to evaluate the use of wound swab samples for the analysis of biomarkers and disease patterns in people with chronic wounds. A sample cohort from the multicenter "Wound-BIOME" project was analyzed. The project aims to comprehensively investigate the local micro-environment of chronic wounds of various entities, healing tendencies and regeneration stages at the biomolecular level. A sample collection and handling protocol suitable for everyday use was tested and evaluated regarding feasibility for multiplex immunoassay, proteomics, small RNA sequencing (miRNA) and metagenome analyses (microbiomics). It could be shown that standard wound swabs are well-suited for the analysis of the complex wound micro-environment using various high-throughput methods. Despite the sample heterogeneity, the quality was adequate to analyze biomolecular patterns. Initial analyses of protein signatures, microbial wound communities and miRNA patterns show promising results for future individualized diagnostics and targeted interventions.
针对患者的诊断和治疗方法在慢性伤口患者的护理中很重要。潜在疾病谱的异质性和伤口微环境的多样性使得通用方法难以实施。虽然高通量分子诊断方法越来越广泛且可用，但对客观生物分子疾病模式的分析尚未应用于日常伤口管理。本研究的目的是评估伤口拭子样本在分析慢性伤口患者生物标志物和疾病模式中的应用。对来自多中心“伤口生物群落”项目的样本队列进行了分析。该项目旨在在生物分子水平上全面研究各种类型、愈合趋势和再生阶段的慢性伤口的局部微环境。测试并评估了一种适用于日常使用的样本采集和处理方案在多重免疫测定、蛋白质组学、小RNA测序（miRNA）和宏基因组分析（微生物组学）方面的可行性。结果表明，标准伤口拭子非常适合使用各种高通量方法分析复杂的伤口微环境。尽管样本存在异质性，但质量足以分析生物分子模式。对蛋白质特征、伤口微生物群落和miRNA模式的初步分析为未来的个性化诊断和靶向干预显示出了有前景的结果。
