Begum Habeebunnisa, Murugesan Periyasamy, Tangutur Anjana Devi
Department of Applied Biology, CSIR-Indian Institute of Chemical Technology, Hyderabad, 500007, India.
Academy of Scientific & Innovative Research, Ghaziabad, Uttar Pradesh, 201002, India.
Biotechniques. 2022 Jun;73(1):58-69. doi: 10.2144/btn-2022-0003. Epub 2022 Jul 1.
Western blotting (WB), also known as immunoblotting, is a well-known molecular biology method that biologists often use to investigate many features of the protein, ranging from basic protein analysis to disease detection. WB is simple, unique, rapid, widely used routine tool with easy interpretation and definite results. It is being used in various fields of science, research and development, diagnostic labs and hospitals. The principle of WB is to accomplish the separation of proteins based on molecular weight and charge. This review addresses in detail the individual steps involved in the WB technique, its troubleshooting, internal loading controls, total protein staining and its diverse applications in scientific research and clinical settings, along with its future perspectives.
蛋白质免疫印迹法（WB），也称为免疫印迹，是一种著名的分子生物学方法，生物学家经常使用它来研究蛋白质的许多特性，从基本的蛋白质分析到疾病检测。WB简单、独特、快速，是一种广泛使用的常规工具，结果易于解读且明确。它被应用于科学、研发、诊断实验室和医院的各个领域。WB的原理是基于分子量和电荷实现蛋白质的分离。本综述详细阐述了WB技术所涉及的各个步骤、故障排除、内参对照、总蛋白染色及其在科研和临床环境中的各种应用，以及其未来展望。
