Sun Dan-Dan, Li Xiao-Qin, Liu Yong-Tong, Ge Meng-Qi, Hou Zhuo-Cheng
National Engineering Laboratory for Animal Breeding, Key Laboratory of Animal Genetics, Breeding and Reproduction of the Ministry of Agriculture, College of Animal Science and Technology, China Agricultural University, Beijing 100193, China.
Animals (Basel). 2024 Oct 15;14(20):2973. doi: 10.3390/ani14202973.
The duck embryo fibroblast cell line CCL-141, which is currently the only commercialized duck cell line, has been underexplored in adipogenesis research. (1) Background: This study establishes an experimental protocol to induce adipogenesis in CCL-141 cells, addressing the importance of understanding gene functions in this process. (2) Methods: Chicken serum, fatty acids, insulin, and all-trans retinoic acid were used to treat CCL-141 cells, with adipogenesis confirmed by Oil Red O staining and gene expression quantification. CRISPR/Cas9 technology was applied to knockout , and the resulting adipogenic phenotype was assessed. (3) Results: The treatments promoted adipogenesis, and the knockout of validated the cell line's utility for gene function studies. (4) Conclusions: CCL-141 cells are a suitable model for investigating duck adipogenesis, contributing to the understanding of regulatory factors in this biological process.
鸭胚胎成纤维细胞系CCL-141是目前唯一商业化的鸭细胞系，在脂肪生成研究中尚未得到充分探索。（1）背景：本研究建立了一种在CCL-141细胞中诱导脂肪生成的实验方案，强调了了解该过程中基因功能的重要性。（2）方法：使用鸡血清、脂肪酸、胰岛素和全反式维甲酸处理CCL-141细胞，通过油红O染色和基因表达定量来确认脂肪生成。应用CRISPR/Cas9技术敲除 ，并评估由此产生的脂肪生成表型。（3）结果：这些处理促进了脂肪生成， 基因的敲除验证了该细胞系在基因功能研究中的实用性。（4）结论：CCL-141细胞是研究鸭脂肪生成的合适模型，有助于理解这一生物学过程中的调控因子。
