Yoo Donghyeok, Oh Minha, Kim Minkyung, Lee Dongbin
Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University, Jinju 52828, Republic of Korea.
Mbiologic LLC., 18017 Sky Park Circle, Irvine, CA 92614, USA.
Animals (Basel). 2024 Oct 17;14(20):2997. doi: 10.3390/ani14202997.
Demineralized bone matrix (DBM) is a widely used allograft material for bone repair, but its handling properties and retention at defect sites can be challenging. Hydroxyethyl cellulose (HEC) has shown promise as a biocompatible carrier for bone graft materials. This study aimed to evaluate the efficacy of DBM combined with cancellous bone putty formed using HEC as an allograft material for bone regeneration in a canine tibial defect model. Experiments were conducted using dogs with proximal tibial defects. Four groups were compared: empty (control group), DBM + HEC (DH), DBM + cancellous bone + HEC (DCH), and DBM + cancellous bone + calcium phosphate + HEC (DCCH). Radiographic, micro-computed tomography (CT), and histomorphometric evaluations were performed 4 and 8 weeks postoperatively to assess bone regeneration. The Empty group consistently exhibited the lowest levels of bone regeneration throughout the study period, indicating that DBM and cancellous bone with HEC significantly enhanced bone regeneration. At week 4, the DCCH group showed the fastest bone regeneration on radiography and micro-computed tomography. By week 8, the DCH group showed the highest area ratio of new bone among all experimental areas, followed by the DH and DCCH groups. This study demonstrated that HEC significantly enhances the handling, mechanical properties, and osteogenic potential of DBM and cancellous bone grafts, making it a promising carrier for clinical applications in canine allograft models. When mixed with allograft cancellous bone, which has high porosity and mechanical strength, it becomes a promising material offering a more effective and reliable option for bone repair and regeneration.
脱矿骨基质（DBM）是一种广泛用于骨修复的同种异体移植材料，但其操作性能以及在缺损部位的留存情况可能具有挑战性。羟乙基纤维素（HEC）已显示出作为骨移植材料生物相容性载体的潜力。本研究旨在评估在犬胫骨缺损模型中，DBM与使用HEC形成的松质骨糊剂联合作为同种异体移植材料用于骨再生的疗效。实验使用患有胫骨近端缺损的犬进行。比较了四组：空白组（对照组）、DBM + HEC（DH组）、DBM + 松质骨 + HEC（DCH组）以及DBM + 松质骨 + 磷酸钙 + HEC（DCCH组）。在术后4周和8周进行影像学、显微计算机断层扫描（CT）和组织形态计量学评估，以评估骨再生情况。在整个研究期间，空白组始终表现出最低水平的骨再生，这表明DBM和含HEC的松质骨显著增强了骨再生。在第4周时，DCCH组在影像学和显微计算机断层扫描上显示出最快的骨再生。到第8周时，DCH组在所有实验区域中显示出新骨面积比例最高，其次是DH组和DCCH组。本研究表明，HEC显著增强了DBM和松质骨移植物的操作性能、力学性能和成骨潜力，使其成为犬同种异体移植模型临床应用中有前景的载体。当与具有高孔隙率和力学强度的同种异体松质骨混合时，它成为一种有前景的材料，为骨修复和再生提供了更有效且可靠的选择。
