Palmer G H, Barbet A F, Davis W C, McGuire T C
Science. 1986 Mar 14;231(4743):1299-302. doi: 10.1126/science.3945825.
Hemoparasitic diseases are endemic in half the world's livestock production areas and are the greatest obstacle to improved meat, milk, and fiber production in the Third World. The most prevalent of these diseases, anaplasmosis, occurs throughout tropical and subtropical regions and is responsible for 50,000 to 100,000 cattle deaths annually in the United States alone. Despite its prevalence and the severity of the losses, an effective immunoprophylaxis for anaplasmosis has not been developed. A neutralization-sensitive epitope on a surface protein with a molecular weight of 105,000 (Am 105) of the causative rickettsia Anaplasma marginale was identified by monoclonal antibody inhibition of infectivity. This epitope was determined to be common to eight isolates with antigenic, morphologic, and protein structural differences. Cattle immunized with Am 105 purified by immunoaffinity chromatography were protected against challenge with virulent Anaplasma marginale. The identification of Am 105 as bearing isolate-common epitopes capable of inducing protection in immunized cattle provides the basis for the development of an effective subunit vaccine for bovine anaplasmosis.
血液寄生虫病在全球一半的畜牧生产地区呈地方性流行，是第三世界提高肉类、牛奶和纤维产量的最大障碍。这些疾病中最常见的是无浆体病，它遍布热带和亚热带地区，仅在美国每年就导致5万至10万头牛死亡。尽管无浆体病流行且损失严重，但尚未开发出有效的免疫预防方法。通过单克隆抗体抑制感染力，在致病性立克次氏体边缘无浆体的一种分子量为105,000的表面蛋白（Am 105）上鉴定出一个中和敏感表位。该表位被确定为八个具有抗原、形态和蛋白质结构差异的分离株所共有。用免疫亲和层析纯化的Am 105免疫的牛受到了保护，可抵御强毒边缘无浆体的攻击。将Am 105鉴定为具有能在免疫牛中诱导保护作用的分离株共同表位，为开发有效的牛无浆体病亚单位疫苗奠定了基础。
