Marine College, Shandong University, Weihai, 264209, Shandong, People's Republic of China.
State Key Laboratory of Microbial Technology, Shandong University, Qingdao, 266237, Shandong, People's Republic of China.
Arch Microbiol. 2021 Jul;203(5):2419-2424. doi: 10.1007/s00203-021-02254-1. Epub 2021 Mar 4.
A Gram-stain-negative, non-motile, aerobic, rod-shaped and reddish-pigmented bacterium, designated 8A47, was isolated from a marine solar saltern located in Wendeng, PR China. The novel strain 8A47 grows at 20-42 °C, pH 7.0-9.0, and in the presence of 2.0-14.0% (w/v) NaCl. Optimal growth was observed at 37-40 °C, pH 7.5-8.0, and with 4.0-6.0% (w/v) NaCl. The phylogenetic analysis based on 16S rRNA gene sequences revealed that strain 8A47 formed an evolutionary lineage with members of the genus Rhodohalobacter. Strain 8A47 exhibited high level of similarity to Rhodohalobacter barlenses MCCC 1K03442 (94.7%) and Rhodohalobacter halophilus JZ3C29 (93.5%). The major fatty acids were summed feature 3 (C ω7c and/or C ω6c), C and iso-C. The sole respiratory quinone was MK-7. The polar lipids of the new isolate consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, one unidentified lipid, two unidentified phospholipids and three unidentified glycolipids and. The genomic DNA G + C content of the strain 8A47 was 47.7 mol%. Based on its phenotypic, chemotaxonomic, genotypic and genomic characteristics presented in this study, strain 8A47 is considered to represent a novel species of the genus Rhodohalobacter, for which the name Rhodohalobacter mucosus sp. nov. is proposed. The type strain is 8A47 (= KCTC 62603 = MCCC 1H00329).
一株革兰氏阴性、非运动性、需氧、杆状、呈红色的细菌，被命名为 8A47，从中国山东省文登市的一个海洋晒盐场中分离得到。新菌株 8A47 在 20-42°C、pH 值 7.0-9.0 以及 2.0-14.0%（w/v）NaCl 的条件下生长。最佳生长条件为 37-40°C、pH 值 7.5-8.0 和 4.0-6.0%（w/v）NaCl。基于 16S rRNA 基因序列的系统发育分析表明，菌株 8A47 与 Rhodohalobacter 属的成员形成了一个进化分支。菌株 8A47 与 Rhodohalobacter barlenses MCCC 1K03442（94.7%）和 Rhodohalobacter halophilus JZ3C29（93.5%）具有高度相似性。主要脂肪酸为 summed feature 3（C ω7c 和/或 C ω6c）、C 和 iso-C。唯一的呼吸醌为 MK-7。新分离株的极性脂包括二磷脂酰甘油、磷脂酰甘油、磷脂酰乙醇胺、一种未鉴定的脂类、两种未鉴定的磷脂和三种未鉴定的糖脂。该菌株的基因组 DNA G+C 含量为 47.7 mol%。基于其在本研究中呈现的表型、化学分类学、基因型和基因组特征，菌株 8A47 被认为代表了 Rhodohalobacter 属的一个新种，建议将其命名为 Rhodohalobacter mucosus sp. nov.。模式菌株为 8A47（=KCTC 62603=MCCC 1H00329）。
