Laboratory of Algae Cultivation, Aquaculture Department, Center for Agrarian Sciences, Federal University of Santa Catarina, Florianópolis, Santa Catarina, Brazil.
Laboratory of Phycology, Botany Department, Center for Biological Sciences, Federal University of Santa Catarina, Florianópolis, Santa Catarina, Brazil.
J Basic Microbiol. 2023 Dec;63(12):1440-1450. doi: 10.1002/jobm.202300362. Epub 2023 Aug 18.
The present study shows the characterization of the bacterial communities associated with different systems during the cultivation of the microalga Tetradesmus obliquus. For that, sequential cultivation was performed in three different systems: (1) Photobioreactor bench-scale; (2) flat-panel photobioreactor; and (3) thin-layer cascade. Cultures were monitored daily for growth parameters and biomass samples were collected for characterization of bacterial communities using metagenomic. A total of 195,177 reads were produced, resulting in the identification of 72 OTUs. In the grouping of bacterial communities, 3 phyla, 6 classes, 28 families, and 35 taxa were found. The bacteria Brevundimonas and Porphyrobacter had a higher relative abundance compared with other taxa found. These taxa were present in all cultivation systems forming a possible core community. Bacterial communities associated with different cultivation systems of the microalga T. obliquus showed an increase in taxa richness and diversity in the super-intensive and intensive systems.
本研究展示了在培养斜生栅藻过程中与不同系统相关的细菌群落的特征化。为此，在三个不同的系统中进行了连续培养：（1） 光生物反应器中试规模；（2） 平板光生物反应器；和（3） 薄层级联。每天监测培养物的生长参数，并收集生物量样品，通过宏基因组学对细菌群落进行特征化。共产生了 195,177 个读数，鉴定出 72 个 OTUs。在细菌群落的分组中，发现了 3 个门、6 个纲、28 个科和 35 个分类群。与其他发现的分类群相比，Brevundimonas 和 Porphyrobacter 细菌的相对丰度更高。这些分类群存在于所有培养系统中，形成了一个可能的核心群落。与斜生栅藻不同培养系统相关的细菌群落显示，在超密集和密集系统中，分类群丰富度和多样性增加。
