Chiossone Cory, Kapes Tanya, Good Robert, Nims Raymond W, Zhou S Steve
Microbac Laboratories, Inc., Sterling, VA 20164, USA.
Syner-G Biopharma, Boulder, CO 80503, USA.
Microorganisms. 2024 Sep 27;12(10):1964. doi: 10.3390/microorganisms12101964.
Safe containment of infectious poliovirus (PV) within Poliovirus-Essential Facilities (PEFs) will require the implementation of reliable PV-inactivation approaches for decontaminating work surfaces. Such approaches should be demonstrated empirically to display adequate efficacy at the use temperature, and the contact times required should be characterized to ensure efficacy. Such efficacy is judged by the ability of the inactivation approach to completely inactivate any PV deposited, with the demonstrated total log reduction in PV titer being as high as empirically achievable. We screened several approaches for their efficacy in inactivating wild-type PV type 1 Chat strain experimentally deposited on stainless-steel carriers at room temperature. On the basis of the results, we selected two approaches (5000 ppm sodium hypochlorite in water and 95% / ethanol in water) for further characterization for repeatability of efficacy (log reduction in PV titer) and time kinetics of inactivation. We now report that both PV-inactivation approaches, which should be readily available to all PEF laboratories globally, fulfill the expectations expressed above, with 5000 ppm sodium hypochlorite reproducibly causing ≥5.38 log inactivation and 95% ethanol reproducibly causing ≥4.46 log inactivation of PV on stainless-steel surfaces within a 5 min contact time at room temperature.
要在脊髓灰质炎病毒必需设施（PEF）内安全容纳传染性脊髓灰质炎病毒（PV），就需要实施可靠的PV灭活方法来对工作表面进行去污。这些方法应通过实验证明在使用温度下具有足够的效力，并且应确定所需的接触时间以确保效力。这种效力通过灭活方法完全灭活任何沉积的PV的能力来判断，所证明的PV滴度的总对数减少应尽可能高。我们筛选了几种方法，以研究它们对实验性沉积在室温下不锈钢载体上的野生型1型PV Chat株的灭活效果。根据结果，我们选择了两种方法（水中5000 ppm次氯酸钠和水中95%乙醇）进行进一步表征，以研究效力的可重复性（PV滴度的对数减少）和灭活的时间动力学。我们现在报告，这两种PV灭活方法全球所有PEF实验室都应易于获得，它们满足上述期望，在室温下5分钟接触时间内，5000 ppm次氯酸钠可重复导致PV在不锈钢表面的灭活≥5.38对数，95%乙醇可重复导致PV的灭活≥4.46对数。
