杀微生物剂对非多孔表面上埃博拉-马孔达病毒的灭活效果：减少病毒传播的有针对性卫生干预措施。

Efficacy of microbicides for inactivation of Ebola-Makona virus on a non-porous surface: a targeted hygiene intervention for reducing virus spread.

机构信息

Applied Biosafety Research Program, Canadian Science Centre for Human and Animal Health, 1015 Arlington Street, Winnipeg, MB, R3E 3P6, Canada.

J. C. Wilt Infectious Diseases Research Centre, Public Health Agency of Canada, 745 Logan Street, Winnipeg, MB, R3E 3L5, Canada.

出版信息

Sci Rep. 2020 Sep 17;10(1):15247. doi: 10.1038/s41598-020-71736-x.

DOI:10.1038/s41598-020-71736-x

PMID:32943689

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7498580/

Abstract

Microbicides play critical roles in infection prevention and control of Ebola virus by decontaminating high-touch environmental surfaces (HITES), interrupting the virus-HITES-hands nexus. We evaluated the efficacy of formulations containing different microbicidal actives for inactivating Ebola virus-Makona strain (EBOV/Mak) on stainless-steel carriers per ASTM E2197-11. Formulations of sodium hypochlorite (NaOCl) (0.05-1%), ethanol (70%), chloroxylenol (PCMX) (0.12-0.48% by weight) in hard water, and a ready-to-use disinfectant spray with 58% ethanol (EDS), were tested at contact times of 0, or 0.5 to 10 min at ambient temperature. EBOV/Mak was inactivated (> 6 log) by 70% ethanol after contact times ≥ 2.5 min, by 0.5% and 1% NaOCl or EDS (> 4 log) at contact times ≥ 5 min, and by 0.12-0.48% PCMX (> 4.2 log) at contact times ≥ 5 min. Residual infectious virus in neutralized samples was assessed by passage on cells and evaluation for viral cytopathic effect. No infectious virus was detected in cells inoculated with EBOV/Mak exposed to NaOCl (0.5% or 1%), PCMX (0.12% to 0.48%), or EDS for ≥ 5 min. These results demonstrate ≥ 6 log inactivation of EBOV/Mak dried on prototypic surfaces by EDS or formulations of NaOCl (≥ 0.5%), PCMX (≥ 0.12%), or 70% ethanol at contact times ≥ 5 min.

摘要

杀菌剂在埃博拉病毒的感染预防和控制中起着关键作用，通过对高接触环境表面（HITES）进行消毒，阻断病毒-HITES-手的接触链。我们按照 ASTM E2197-11 标准，评估了含有不同杀菌剂的配方对不锈钢载体上的埃博拉病毒-Makona 株（EBOV/Mak）的灭活效果。次氯酸钠（NaOCl）（0.05-1%）、乙醇（70%）、对氯间二甲苯酚（PCMX）（0.12-0.48%重量比）在硬水中的配方，以及含有 58%乙醇的即用型消毒喷雾（EDS），在环境温度下的接触时间为 0 分钟或 0.5-10 分钟时进行了测试。70%乙醇接触时间≥2.5 分钟可使 EBOV/Mak 失活（>6 对数），0.5%和 1% NaOCl 或 EDS（>4 对数）在接触时间≥5 分钟时失活，0.12-0.48% PCMX（>4.2 对数）在接触时间≥5 分钟时失活。通过细胞接种和病毒细胞病变效应评估来评估中和样本中残留的传染性病毒。用暴露于 NaOCl（0.5%或 1%）、PCMX（0.12%至 0.48%）或 EDS≥5 分钟的 EBOV/Mak 处理的细胞接种的细胞中未检测到感染性病毒。这些结果表明，EDS 或含有 NaOCl（≥0.5%）、PCMX（≥0.12%）或 70%乙醇的配方在接触时间≥5 分钟时，可使原型表面干燥的 EBOV/Mak 失活≥6 对数。