Eaton William D, Hamilton Debra A
Biology Department, Dyson College, Pace University, New York, NY 10038, USA.
Department of Environment and Development, University for Peace, El Rodeo de Mora, San José 10701, Costa Rica.
Microorganisms. 2024 Sep 30;12(10):1996. doi: 10.3390/microorganisms12101996.
Leguminous trees are thought to enhance soil carbon (C) accumulation following reforestation, through mostly unknown mechanisms. This study amplified soil DNA using the ITS1F and ITS4 primers for PCR and Illumina MiSeq methods to identify fungal taxa, and traditional C analysis methods to evaluate how planted 4-, 8-, and 11-year-old trees affected soil fungal community compositions and C utilization patterns compared to old-growth trees and an adjacent unplanted pasture within the same reforestation zone in Monteverde, Costa Rica. Along the tree age gradient, the planted trees enhanced the tree soil C capture capacity, as indicated by increased levels of soil biomass C, Respiration, and efficiency of organic C use (with lower CO values), and development of increasingly more abundant, stable, and successionally developed fungal communities, including those associated with the decomposition of complex organic C compounds. The level and strength of differences coincided with differences in the time of separation between the pasture and tree age or between the different tree ages. Fungal taxa were also identified as potential indicators of the early and late stages of soil recovery. Thus, planting should be part of future reforestation strategies used in this region of the Monteverde Cloud Forest in Costa Rica.
豆科树木被认为在重新造林后能通过大多未知的机制增强土壤碳（C）积累。本研究使用ITS1F和ITS4引物通过聚合酶链式反应（PCR）和Illumina MiSeq方法扩增土壤DNA以鉴定真菌类群，并采用传统的碳分析方法来评估与哥斯达黎加蒙特维多云雾森林同一重新造林区域内的老龄树木和相邻未种植牧场相比，种植4年、8年和11年的树木如何影响土壤真菌群落组成和碳利用模式。沿着树木年龄梯度，种植的树木增强了土壤碳捕获能力，表现为土壤生物量碳水平、呼吸作用以及有机碳利用效率（较低的CO值）的增加，以及真菌群落日益丰富、稳定且按演替发展，包括那些与复杂有机碳化合物分解相关的群落。差异的程度和强度与牧场和树木年龄之间或不同树木年龄之间的分离时间差异一致。真菌类群也被确定为土壤恢复早期和晚期的潜在指标。因此，植树造林应成为哥斯达黎加蒙特维多云雾森林该地区未来重新造林策略的一部分。
