Tang Guangfei, Xia Haoxue, Liang Jingting, Ma Zhonghua, Liu Wende
State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, China.
State Key Laboratory of Rice Biology, Key Laboratory of Molecular Biology of Crop Pathogens and Insects, Institute of Biotechnology, Zhejiang University, Hangzhou, China.
Front Microbiol. 2021 Nov 19;12:765398. doi: 10.3389/fmicb.2021.765398. eCollection 2021.
Putrescine, spermidine, and spermine are the most common natural polyamines. Polyamines are ubiquitous organic cations of low molecular weight and have been well characterized for the cell function and development processes of organisms. However, the physiological functions of polyamines remain largely obscure in plant pathogenic fungi. causes Fusarium head blight (FHB) and leads to devastating yield losses and quality reduction by producing various kinds of mycotoxins. Herein, we genetically analyzed the gene function of the polyamine biosynthesis pathway and evaluated the role of the endogenous polyamines in the growth, development, and virulence of . Our results found that deletion of spermidine biosynthesis gene caused serious growth defects, reduced asexual and sexual reproduction, and increased sensitivity to various stresses. More importantly, Δ exhibited significantly decreased mycotoxin deoxynivalenol (DON) production and weak virulence in host plants. Additionally, the growth and virulence defects of Δ could be rescued by exogenous application of 5 mM spermidine. Furthermore, RNA-seq displayed that FgSpe3 participated in many essential biological pathways including DNA, RNA, and ribosome synthetic process. To our knowledge, these results indicate that spermidine is essential for growth, development, DON production, and virulence in species, which provides a potential target to control FHB.
腐胺、亚精胺和精胺是最常见的天然多胺。多胺是普遍存在的低分子量有机阳离子，其在生物体的细胞功能和发育过程中已得到充分表征。然而，多胺在植物病原真菌中的生理功能仍 largely 不清楚。 引起小麦赤霉病（FHB），并通过产生各种霉菌毒素导致毁灭性的产量损失和品质下降。在此，我们对多胺生物合成途径的基因功能进行了遗传分析，并评估了内源性多胺在 的生长、发育和毒力中的作用。我们的结果发现，亚精胺生物合成基因 的缺失导致严重的生长缺陷，无性和有性繁殖减少，并增加了对各种胁迫的敏感性。更重要的是，Δ在宿主植物中表现出显著降低的霉菌毒素脱氧雪腐镰刀菌烯醇（DON）产量和弱毒力。此外，外源施加5 mM亚精胺可以挽救Δ的生长和毒力缺陷。此外，RNA测序显示FgSpe3参与了许多重要的生物学途径，包括DNA、RNA和核糖体合成过程。据我们所知，这些结果表明亚精胺对于 物种的生长、发育、DON产生和毒力至关重要，这为控制FHB提供了一个潜在靶点。
